h**********r
发帖数: 671 | 1 以前好好的。接着几次跑胶小片段都有点弥散，不知道原因。左图是筛克隆时的双酶切
，质粒是按《分子克隆》的碱裂解法提的。右图借用别的lab的qiagen的试剂盒提的然
后双酶切。问题也可能出在loading dye上。按《分子克隆》配的6X,40% sucrose加上
两种染料。EB是直接加在胶里的。大家看看吧，多谢！ | Z******5
发帖数: 435 | | h**********r
发帖数: 671 | | w*****n
发帖数: 107 | 4 There is no problem with you gel and running method, because the markers
gave you very sharp bands.
Check the restriction enzyme you use, and make sure the DNA you digested do
not have multiply sites close to each other. |
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