s******y
发帖数: 220 | 1 I was told it's OK to reuse once, or even twice, but never tried, is it
common for people to reuse the WB transfer buffer?
Thanks. |
i*****n
发帖数: 53 | 2 no,
RUNNING BUFFER, YES. |
C*******e
发帖数: 4348 | 3 以前不reuse
新实验室的人reuse
好像没啥不妥 |
y**u
发帖数: 7459 | 4 no, we don't reuse any buffer, but I know some people do and it seems to be
fine
【在 s******y 的大作中提到】
: I was told it's OK to reuse once, or even twice, but never tried, is it
: common for people to reuse the WB transfer buffer?
: Thanks.
|
h*******o
发帖数: 4884 | 5 I do it all the time. It should be fine to reuse for a couple of times.
Sometimes, I added a little methanol to the old buffer. |
H*g
发帖数: 2333 | 6 Only reuse for one time. |
v***a
发帖数: 1242 | 7 可以的。我们一般用3次
【在 s******y 的大作中提到】
: I was told it's OK to reuse once, or even twice, but never tried, is it
: common for people to reuse the WB transfer buffer?
: Thanks.
|
C***Y
发帖数: 61 | 8 I usually reuse transfer buffer more than 10 times, and never had problem so
far. |
h*******n
发帖数: 2052 | 9 NB!
so
【在 C***Y 的大作中提到】
: I usually reuse transfer buffer more than 10 times, and never had problem so
: far.
|
y******8
发帖数: 1764 | 10 you can reuse the transfer buffer untill the resistance drops to a certain
point. My experience, up to five times. |
l*********1
发帖数: 351 | 11 好像还有篇journal讲你能重复使用wb buffer五次以上,毕竟有methanol,环保环保. |
n******e
发帖数: 89 | 12 我是一直用，每次用损耗一些，过几次补些新的。平常都放得很冷，低温下做电流低，
溶液寿命更长。
这是常规的转膜，如果是半干式的，每次用量很少，就无所谓了，用完就扔掉。
problem so
【在 C***Y 的大作中提到】
: I usually reuse transfer buffer more than 10 times, and never had problem so
: far.
|
