g*********3
发帖数: 177 | 1 小弟第一次发帖，请教下各位~
如果要进行liver metabolic flux analysis，现在比较通用的技术是？这些技术是否
够sensitive能够反应诸如pyruvate,cAMP,glucose等等这些比较重要metabolites的变
化。
谢谢各位。 | C*********u
发帖数: 811 | 2 seahorse
【在 g*********3 的大作中提到】
: 小弟第一次发帖，请教下各位~
: 如果要进行liver metabolic flux analysis，现在比较通用的技术是？这些技术是否
: 够sensitive能够反应诸如pyruvate,cAMP,glucose等等这些比较重要metabolites的变
: 化。
: 谢谢各位。
| h*******o
发帖数: 4884 | 3 Seahorse is for in vitro, not in vivo.
The best seahorse can do so far (in addition to cell culture) is on
isolated islets, or brain slices, synaptosome, or isolated mitochondria
【在 C*********u 的大作中提到】
: seahorse
| C*********u
发帖数: 811 | 4 yes, it is for in vitro.
For in vivo, I think it will be very tough to measure. Using primary
cultured cells could be a comprimised way.
【在 h*******o 的大作中提到】
: Seahorse is for in vitro, not in vivo.
: The best seahorse can do so far (in addition to cell culture) is on
: isolated islets, or brain slices, synaptosome, or isolated mitochondria
| n***w
发帖数: 2405 | 5 so how much is it for a whole set of measurements?
7k? | h*******o
发帖数: 4884 | 6 at least X10 times for the XF 24 system
plus all the consumables.
But it is worth every penny if you know what to look for using the system.
【在 n***w 的大作中提到】
: so how much is it for a whole set of measurements?
: 7k?
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