k***g
发帖数: 4904 | 1 只是普适性的对样品板上存在的细菌（2um左右大小的）进行染色，然后进行显微镜观
察，用什么方法最为方便？有染料可以无差别的对各种细菌进行染色，达到足以显微镜
目测的程度吗？用荧光染色的话，最方便廉价的方法是什么呢？谢谢啦！ |
k***g
发帖数: 4904 | |
J*********o
发帖数: 302 | 3 根本不用染色，直接看。只是注意相差的显微镜就好了 |
J*********o
发帖数: 302 | 4 根本不用染色，直接看。只是注意相差的显微镜就好了 |
c****l
发帖数: 1086 | 5 Gram stain is the most common one. Others like acid fast, silver etc
depending on what your bugs are. |
k***g
发帖数: 4904 | 6 我的substrate不是平的，用phase contrast应该也没法分辨。谢谢楼上几位！ |
k***g
发帖数: 4904 | 7 Thanks. I just want to dye the microbes to visualize them; no need to
differentiate or identify them. Is there any general and simple method to do
that? Also, is there any general method to label the bacteria with
fluorescent dye?
【在 c****l 的大作中提到】
: Gram stain is the most common one. Others like acid fast, silver etc
: depending on what your bugs are.
|
e**r
发帖数: 1144 | 8 结晶紫
do
【在 k***g 的大作中提到】
: Thanks. I just want to dye the microbes to visualize them; no need to
: differentiate or identify them. Is there any general and simple method to do
: that? Also, is there any general method to label the bacteria with
: fluorescent dye?
|
k***g
发帖数: 4904 | |
b****o
发帖数: 193 | 10 Gram-stain
DAPI
FISH比较麻烦，用细菌通用荧光探针Eu-388. |
