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Biology版 - aske for help
相关主题
【求助】救命啊,细胞为什么死掉了,在线等anybody work or know people in Whitehead institue?
求救!已经小心得不能再小心了,为什么还有contamination?//包子伺候!How to purify DNA contaminated with polysaccharides?
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Re: Please give me some encouragementdo you autoclave tubs and tips used for PCR?
用flask和dish培养细胞有什么区别?PCR空白对照有扩增,并且熔解曲线是单峰说明什么?
mycoplasma infection的问题克隆不长colony是啥原因呢?
困惑,knock down在蛋白水平work,但qpcr检测不到差别求救,全实验室的cell line都被mycoplasma污染了
相关话题的讨论汇总
话题: aske话题: anyone话题: tell话题: could
进入Biology版参与讨论
1 (共1页)
i**g
发帖数: 2261
1
anyone could tell me what the contamination is and how to deal with it?
Thanks a lot!
s******s
发帖数: 13035
2
细胞长菌了? 不是一定要save的还是扔了重做把。一定要留下的,
就多用pbs洗N遍,然后用5x gentamycin处理一下午,然后泡在1x里面,
每天早上来看看,洗两遍,换gentamycin medium,搞个一个礼拜试试
不保证work

【在 i**g 的大作中提到】
: anyone could tell me what the contamination is and how to deal with it?
: Thanks a lot!

i**g
发帖数: 2261
3
Thanks. same contamination happened several times... I don't know the reason
..

【在 s******s 的大作中提到】
: 细胞长菌了? 不是一定要save的还是扔了重做把。一定要留下的,
: 就多用pbs洗N遍,然后用5x gentamycin处理一下午,然后泡在1x里面,
: 每天早上来看看,洗两遍,换gentamycin medium,搞个一个礼拜试试
: 不保证work

L*******e
发帖数: 2153
4
http://www.invitrogen.com/site/us/en/home/References/gibco-cell
try this one.

【在 i**g 的大作中提到】
: anyone could tell me what the contamination is and how to deal with it?
: Thanks a lot!

1 (共1页)
进入Biology版参与讨论
相关主题
求救,全实验室的cell line都被mycoplasma污染了Re: Please give me some encouragement
help!!!!! about the cell culture用flask和dish培养细胞有什么区别?
什么浓度的琼脂糖胶能够把2200bp和2000bp的DNA片段分开?mycoplasma infection的问题
paper needed困惑,knock down在蛋白水平work,但qpcr检测不到差别
【求助】救命啊,细胞为什么死掉了,在线等anybody work or know people in Whitehead institue?
求救!已经小心得不能再小心了,为什么还有contamination?//包子伺候!How to purify DNA contaminated with polysaccharides?
Re: how to avoid contamination when doing PCR?帮忙troubleshooting一下我的culture contamination问题
Re: cloning help!Murder By Injection: The Story Of The Medical Conspiracy Ag (转载)
相关话题的讨论汇总
话题: aske话题: anyone话题: tell话题: could