l*****3
发帖数: 12 | 1 不就是artificial enzyme complex么，09年nbt keasling那片，后来igem的nas，然后
vt zhang的angew都是这个套路么，这不就用个polymer代替下docker分子么？
Nat Nanotechnol. 2013 Feb 17. doi: 10.1038/nnano.2012.264. [Epub ahead of
print]
Biomimetic enzyme nanocomplexes and their use as antidotes and preventive
measures for alcohol intoxication.
Liu Y, Du J, Yan M, Lau MY, Hu J, Han H, Yang OO, Liang S, Wei W, Wang H, Li
J, Zhu X, Shi L, Chen W, Ji C, Lu Y.
Source
1] Key Laboratory of Functional Polymer Materials, Ministry of Education,
and Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China
[2] Department of Chemical and Biomolecular Engineering, University of
California, Los Angeles, California 90095, USA [3].
Organisms have sophisticated subcellular compartments containing enzymes
that function in tandem. These confined compartments ensure effective
chemical transformation and transport of molecules, and the elimination of
toxic metabolic wastes. Creating functional enzyme complexes that are
confined in a similar way remains challenging. Here we show that two or more
enzymes with complementary functions can be assembled and encapsulated
within a thin polymer shell to form enzyme nanocomplexes. These
nanocomplexes exhibit improved catalytic efficiency and enhanced stability
when compared with free enzymes. Furthermore, the co-localized enzymes
display complementary functions, whereby toxic intermediates generated by
one enzyme can be promptly eliminated by another enzyme. We show that
nanocomplexes containing alcohol oxidase and catalase could reduce blood
alcohol levels in intoxicated mice, offering an alternative antidote and
prophylactic for alcohol intoxication. |
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