d********r
发帖数: 211 | 1 http://www.ncbi.nlm.nih.gov/pubmed/21832238
见附图. 我的疑问是: T cell 都被CD3/CD28 beads包围了, 这时候来做lentivirus
transduction, 不是明显减少了很多细胞的表面来供viral vector进攻吗? 这不会影响
transduction efficiency吗? |
S*****s
发帖数: 242 | 2 The bead should be washed out before putting into bag.
【在 d********r 的大作中提到】
: http://www.ncbi.nlm.nih.gov/pubmed/21832238
: 见附图. 我的疑问是: T cell 都被CD3/CD28 beads包围了, 这时候来做lentivirus
: transduction, 不是明显减少了很多细胞的表面来供viral vector进攻吗? 这不会影响
: transduction efficiency吗?
|
d********r
发帖数: 211 | 3 are you sure? 见附图, harvest day 才remove beads啊.
【在 S*****s 的大作中提到】
: The bead should be washed out before putting into bag.
|
d********r
发帖数: 211 | 4 还是这篇文章, 他们怎么把successfully transfected cells 和 non-transfected
cells 分开的啊?
【在 d********r 的大作中提到】
: http://www.ncbi.nlm.nih.gov/pubmed/21832238
: 见附图. 我的疑问是: T cell 都被CD3/CD28 beads包围了, 这时候来做lentivirus
: transduction, 不是明显减少了很多细胞的表面来供viral vector进攻吗? 这不会影响
: transduction efficiency吗?
|
l********7
发帖数: 175 | 5 不用分，临床已经够用的了。这玩艺在体内可以扩增。不需要太高的转化效率。 |
d*p
发帖数: 534 | 6 Beads are much smaller than T cells. 1:1 ratio is good enough to activate T
cells. I suggest you learn what T cell synapse is, if you don't have
immunology background. |
d********r
发帖数: 211 | 7 CD3/CD28 beads are 4.5 μm diameter, not too small. (see in the link below)
http://products.invitrogen.com/ivgn/product/11161D
Also I think they are using 3:1 ratio.
Some information suggests that transduction may happen better when the cells
are being activated, so they may want to have the beads there...
Not sure...
T
【在 d*p 的大作中提到】
: Beads are much smaller than T cells. 1:1 ratio is good enough to activate T
: cells. I suggest you learn what T cell synapse is, if you don't have
: immunology background.
|
