m**********3
发帖数: 706 | 1 今天查文献，关键词搜索" survival，retinal neurons". 有一篇文章让我很迷惑。仔
细一看名字和地址，莫非是气功大师的杰作。求鉴定。如果真是，那严大师应该是所有
这些大师，教主里头科研素质最高的了。放在国内都可以PHD毕业了。甚至秒杀大部分
博导.
Int J Neurosci. 2002 Oct;112(10):1209-27.
XY99-5038 promotes long-term survival of cultured retinal neurons.
Yan X, Fong YT, Wolf D, Shen H, Zaharia M, Wang J, Wolf G, Li F, Lee GD, Cao
W.
Source
Institute of Chongqing Traditional Chinese Medicine, Chongqing, Sichuang, P.
R. of China.
Abstract
We have previously reported that XY99-5038, a preparation from a specific
formula of Traditional Chinese Medicine, could effectively inhibit hydrogen
peroxide-induced retinal cell death. In the present study, we investigated
the possibility of XY99-5038 to prolong neuronal survival in a long-term
retinal neuronal culture. Basic fibroblast survival factor (bFGF), a potent
neurotrophic factor, was employed as comparable agent. Retinas of 0-2 days
old Sprague-Dawley rats were isolated and dissociated. The cells were
maintained in tissue culture for up to 9 weeks in a synthetic serum-free
media. XY99-5038 (100 ng/ml) or a vehicle was added to culture every 3-4
days, starting at the first week of culturing. The number of cells were
counted and compared for each time point and treatment. Cell viability was
also determined by MTT assay, whereas apoptotic cell death was evaluated by
the TUNEL assay. XY99-5038 treatment significantly reduced cell loss,
increased cell viability, and inhibited apoptosis in this long-term retinal
neuronal culture. Our data also show that the protective effect of XY99-5038
is more potent than that of bFGF. Our data suggest that XY99-5038 could be
beneficial to the prolongation of neuron survival.
貌似这篇也是的，查了影响因子，居然还有2
Protective effect of XY99-5038 on hydrogen peroxide induced cell death in
cultured retinal neurons.
Life Sci. 2001 Jun 8;69(3):289-99.
Xin Y, Fong YT, Wolf G, Wolf D, Cao W.
The Institute of Chongchi Traditional Chinese Medicine, Chongchi, Sichuang,
People's Republic of China.
Abstract
XY99-5038 is a preparation from a specific formula provided by Dr. Yan Xin,
a renowned Traditional Chinese Medicine specialist. This formulation has
proven to be effective without side effects but has not been studied under
rigorous laboratory conditions. In order to investigate the role of XY99-
5038 in protecting neurons from degeneration, we used retinal neuronal
culture as a model system to study the protective effects of XY99-5038
against hydrogen peroxide (H2O2) induced cytotoxicity. Basic fibroblast
growth factor (bFGF), a potent neurotrophic factor, was employed as
comparable agent. Retinas of 0-2 days old Sprague-Dawley rats were isolated,
dissociated, and the neurons maintained for 2 weeks in a synthetic serum-
free media. Cytotoxicity was determined by MTT (3,(4,5-dimethylthiazol-2-yl)
2,5-diphenyl-tetrazolium bromide) and LDH (Lactate dehydrogenase) assays,
whereas apoptotic cell death was evaluated by the TUNEL (TdT-mediated
digoxigenin-dUTP nick-end labeling) assay. Treatment with H2O2 significantly
induced death of retinal neurons. Pretreatment with XY99-5038 prior to
insult greatly inhibited or attenuated H2O2 induced cytotoxicity and
apoptosis. These effects were shown to be dose-dependent. Our data also show
that the protective effect of XY99-5038 is more potent than that of bFGF.
Our data suggest that XY99-5038 could be a potential agent in the treatment
of disorders associated with oxidative damage.
PMID: 11441920 [PubMed - indexed for MEDLINE] | m*******n
发帖数: 387 | | m**********3
发帖数: 706 | 3 Niu!
sheng
【在 m*******n 的大作中提到】
: https://sites.google.com/site/yanxinqigong/Home/qi-gong-ke-yan/yan-xin-sheng
: -ming-ke-ji-xin-xi
: 给你贴个中文介绍
: 估计是同一个人
: 不知道那个XY99-5038 是个什么神奇的东西？
| a*********n
发帖数: 2526 | 4 岩心做事情还是很认真
Cao
P.
【在 m**********3 的大作中提到】
: 今天查文献，关键词搜索" survival，retinal neurons". 有一篇文章让我很迷惑。仔
: 细一看名字和地址，莫非是气功大师的杰作。求鉴定。如果真是，那严大师应该是所有
: 这些大师，教主里头科研素质最高的了。放在国内都可以PHD毕业了。甚至秒杀大部分
: 博导.
: Int J Neurosci. 2002 Oct;112(10):1209-27.
: XY99-5038 promotes long-term survival of cultured retinal neurons.
: Yan X, Fong YT, Wolf D, Shen H, Zaharia M, Wang J, Wolf G, Li F, Lee GD, Cao
: W.
: Source
: Institute of Chongqing Traditional Chinese Medicine, Chongqing, Sichuang, P.
| e**f
发帖数: 144 | 5 还有几篇呢
1: Yan X, Shen H, Jiang H, Hu D, Wang J, Wu X. External Qi of Yan Xin Qigong
inhibits activation of Akt, Erk1/2 and NF-?B and induces cell cycle arrest a
nd
apoptosis in colorectal cancer cells. Cell Physiol Biochem. 2013;31(1):113-2
2.
doi: 10.1159/000343354. Epub 2013 Jan 25. PubMed PMID: 23363659.
2: Yan X, Li F, Dozmorov I, Frank MB, Dao M, Centola M, Cao W, Hu D. Externa
l Qi
of Yan Xin Qigong induces cell death and gene expression alterations promoti
ng
apoptosis and inhibiting proliferation, migration and glucose metabolism in
small-cell lung cancer cells. Mol Cell Biochem. 2012 Apr;363(1-2):245-55. do
i:
10.1007/s11010-011-1176-8. Epub 2011 Dec 10. PubMed PMID: 22160803; PubMed
Central PMCID: PMC3567610.
3: Yan X, Shen H, Jiang H, Hu D, Zhang C, Wang J, Wu X. External Qi of Yan X
in
Qigong Induces apoptosis and inhibits migration and invasion of
estrogen-independent breast cancer cells through suppression of Akt/NF-kB
signaling. Cell Physiol Biochem. 2010;25(2-3):263-70. doi: 10.1159/000276560
.
Epub 2010 Jan 12. PubMed PMID: 20110687.
4: Yan X, Shen H, Jiang H, Zhang C, Hu D, Wang J, Wu X. External Qi of Yan X
in
Qigong induces G2/M arrest and apoptosis of androgen-independent prostate ca
ncer
cells by inhibiting Akt and NF-kappa B pathways. Mol Cell Biochem. 2008
Mar;310(1-2):227-34. Epub 2007 Dec 16. PubMed PMID: 18080802.
5: Yan X, Shen H, Jiang H, Zhang C, Hu D, Wang J, Wu X. External Qi of Yan X
in
Qigong differentially regulates the Akt and extracellular signal-regulated k
inase
pathways and is cytotoxic to cancer cells but not to normal cells. Int J Bio
chem
Cell Biol. 2006;38(12):2102-13. Epub 2006 Jun 27. PubMed PMID: 16893670.
Cao
P.
【在 m**********3 的大作中提到】
: 今天查文献，关键词搜索" survival，retinal neurons". 有一篇文章让我很迷惑。仔
: 细一看名字和地址，莫非是气功大师的杰作。求鉴定。如果真是，那严大师应该是所有
: 这些大师，教主里头科研素质最高的了。放在国内都可以PHD毕业了。甚至秒杀大部分
: 博导.
: Int J Neurosci. 2002 Oct;112(10):1209-27.
: XY99-5038 promotes long-term survival of cultured retinal neurons.
: Yan X, Fong YT, Wolf D, Shen H, Zaharia M, Wang J, Wolf G, Li F, Lee GD, Cao
: W.
: Source
: Institute of Chongqing Traditional Chinese Medicine, Chongqing, Sichuang, P.
| e*****i
发帖数: 149 | | k***g
发帖数: 4904 | 7 MTT验证中药成分对细胞的作用，算是很简单的思路和操作吧，他们发个文章也不算不
可能吧
Cao
P.
【在 m**********3 的大作中提到】
: 今天查文献，关键词搜索" survival，retinal neurons". 有一篇文章让我很迷惑。仔
: 细一看名字和地址，莫非是气功大师的杰作。求鉴定。如果真是，那严大师应该是所有
: 这些大师，教主里头科研素质最高的了。放在国内都可以PHD毕业了。甚至秒杀大部分
: 博导.
: Int J Neurosci. 2002 Oct;112(10):1209-27.
: XY99-5038 promotes long-term survival of cultured retinal neurons.
: Yan X, Fong YT, Wolf D, Shen H, Zaharia M, Wang J, Wolf G, Li F, Lee GD, Cao
: W.
: Source
: Institute of Chongqing Traditional Chinese Medicine, Chongqing, Sichuang, P.
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