x******m
发帖数: 736 | 1 I have RNA-seq data from Illumina GA (single-ended)and Hiseq (pair-ended).
Just wonder if I could put them together in differential gene expression
analysis. I know pair-end is much better than single-end in accuracy. But
not sure if it is reasonable to put them together in the analysis. many
thanks |
t******g
发帖数: 372 | 2 u can, as long as u have the lib type accounted for
check the package vignette for a similar analysis
【在 x******m 的大作中提到】
: I have RNA-seq data from Illumina GA (single-ended)and Hiseq (pair-ended).
: Just wonder if I could put them together in differential gene expression
: analysis. I know pair-end is much better than single-end in accuracy. But
: not sure if it is reasonable to put them together in the analysis. many
: thanks
|
x******m
发帖数: 736 | 3 thanks for reply.
cannot find the exact tutorial for mixed single and pair-ended cases. Do you
mind show me one? many thanks. |
k*****n
发帖数: 323 | 4 可以啊，你把paired去掉一边的read就好了 |
x******m
发帖数: 736 | 5 哈哈，这个主意牛B。
【在 k*****n 的大作中提到】
: 可以啊，你把paired去掉一边的read就好了
|
