r**********e
发帖数: 587 | 1 I don't know anyone here has experience with plasmid transfection fir SH-
SY5Y?
Lipofectamine 3000 doesn't work at all.
Neuronal line is always challenging for transfection.
Any recommendations of good reagent to achieve relatively high transfection
efficiency?
Thx! |
d******n
发帖数: 3836 | |
j*****9
发帖数: 716 | 3 I have tried about 10 lipid-based transfection reagents in SH-SY5Y and
neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or
Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS).
Good luck
transfection
【在 r**********e 的大作中提到】
: I don't know anyone here has experience with plasmid transfection fir SH-
: SY5Y?
: Lipofectamine 3000 doesn't work at all.
: Neuronal line is always challenging for transfection.
: Any recommendations of good reagent to achieve relatively high transfection
: efficiency?
: Thx!
|
U*****e
发帖数: 40 | 4 我们公司自己开发了一种转染试剂，同现在市售的转染试剂都不同，比较广谱，相对各
种核酸及细胞都可以用。而且可以用荧光实时检测转染试剂同质粒符合物的生成及转染
细胞的过程。产品还没用放入公司网页。如果你想试试，我们可以提供试用样品。
Bioandbeyond.com |
c**i
发帖数: 265 | 5 我们lab用FuGene HD，效率一般。现在准备做lenti |
c*******s
发帖数: 29 | 6 Xfect from Clontech is relatively the best in our hand.
transfection
【在 r**********e 的大作中提到】
: I don't know anyone here has experience with plasmid transfection fir SH-
: SY5Y?
: Lipofectamine 3000 doesn't work at all.
: Neuronal line is always challenging for transfection.
: Any recommendations of good reagent to achieve relatively high transfection
: efficiency?
: Thx!
|
s*****g
发帖数: 7857 | 7 lonza电转
即使用其他公司试剂(Mirus)也行
我曾经也面对过难转染的细胞 |
U*****e
发帖数: 40 | 8 Updated link: visible transfection
http://bioandbeyond.com/p/whats-new |
r**********e
发帖数: 587 | 9 Hi Thanks
For NeuroPORTER
Genlantis or Sigma-Aldrich, which one is better? And why the same product
but two companies? And I don't see Genlantis is being merged by Sigma
or
【在 j*****9 的大作中提到】
: I have tried about 10 lipid-based transfection reagents in SH-SY5Y and
: neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or
: Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS).
: Good luck
:
: transfection
|
