y****6
发帖数: 196 | 1 I need to digest an antibody into small fragments. Which protease is the
best? Trypsin?
Thanks a lot |
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c******g
发帖数: 49 | 2 谢谢。能不能推荐个rabbit antibody? |
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l****j
发帖数: 70 | 3 Epitomics是属于Abcam的公司，有过他们rabbit monoclonal antibody订制产品的吗？
效果怎么样？ |
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b**********8
发帖数: 349 | 4 正郁闷呢，定了他家的一个rabbit monoclonal antibody，看了下比Abcam便宜了不到
100刀，无比悲催地等待了一个月还没收到货，还在继续等待中 |
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a*********n
发帖数: 2526 | 5 abcam 公司的antibody 又贵又没有质量保证 |
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a*********n
发帖数: 2526 | 6 no kidding
reused antibody has low background |
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I********w
发帖数: 763 | 7 最近要做一个课题， 需要先找家公司做antibody affinity maturation screening.
网上一搜， 很多家都提供这项服务，不了解行情, 不知道哪家靠谱。请问本版有没有
xdjm有相关经验？ 谢谢！ |
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h***a
发帖数: 145 | 8 Which company's Ab is good for ELISA? Need biotin conugated anti mouse IgA,
IgG1, And IgG2a antibodies.
Thanks in advance! |
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p*****h
发帖数: 36 | 9 Ditto this.
We bought a tube of Sigma M2 antibody this Feb. It didn't work at all (no
bands whatsoever). Earlier batches done side by side worked well. |
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z********8
发帖数: 818 | 10 Stratgene Flag antibody很好。 |
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g*********5
发帖数: 2533 | 11 Just bought this one,
sucks.
do someone know good flag tag antibody from other company? |
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d****i
发帖数: 2346 | 12
这么杯具？愁死了，我也需要一个flag antibody，难道真的需要一个一个试过去？ |
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s******y
发帖数: 28562 | 13 Genscript
They even have the guarantee service. If the phospho-specific antibody is
not successful they don't charge you. |
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h****k
发帖数: 182 | 14 试了Qiagen的Tetra His antibody 可以用但是巨贵，100ug 六百块钱，求推荐个便宜
的。Thanks! |
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e****s
发帖数: 1125 | 15 做Western吗？ His antibodies没几个适合做Western的。
Qiagen这个能大概检测多少ng的蛋白？ |
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h****k
发帖数: 182 | 16 是用来做western的，网上写的 “The sensitivity of Tetra·His Antibody in
western blots is up to 2 ng, and up to 0.5 ng in dot blots ” |
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d***l
发帖数: 50 | 17 最近需要做一个phospho-specific antibody，请有经验的推荐一个好公司。看到几件
国内的公司非常便宜 （genescripts, abmart），$1000-2000, 美国的都要$5000-$
8000，不知道质量如何。主要是要有经验成功率高的。谢了。 |
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f*********9
发帖数: 258 | 19 abcam's HA antibody is really good. They are 100% guarantee |
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w*********1
发帖数: 27 | 20 Thanks. But I think the antibody from abcam is really expensive too..... |
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w*********1
发帖数: 27 | 21 Thanks. Have you ever tried HA antibody from Covance before and how is that? |
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p********i
发帖数: 116 | 22 用了很多年，非常好。
Thanks. Have you ever tried HA antibody from Covance before and how is that? |
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f***n
发帖数: 34 | 23 I tried some anti-HA antibodies. The one from convace is best. |
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j*****j
发帖数: 39 | 24 If someone can recommend a V5 tag antibody for WB and IP, thank you very
much. |
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s******y
发帖数: 28562 | 25 R96025 ANTI-V5 ANTIBODY 50 UL |
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i*********0
发帖数: 915 | 26 各位牛牛，有没有好用点的antibody可以推荐的。
准备sorting一下HSC，然后做个IF staining。
谢谢！ |
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w********y
发帖数: 288 | 27 想买一个抗体，在anbibody resource上搜了一下，发现好多公司都有，不过仔细看产
品信息，应该是同一种，猜测应该一家是原厂，其他都是代理。价格各不相同，我不知
道这种情况应该怎么选，是找一个便宜的买就行，还是应该从原厂买更放心。如果原厂
的好，怎么知道谁是呢？试着打了几个客服，都含糊其辞，好像不愿意说似的。
我平时antibody用的少，不太有经验，想来版上请教一下，这种情况应该怎么选，谢谢
。 |
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H*****e
发帖数: 120 | 28 Thanks! This is what I am wondering. We had mouse tumor samples and we had
determine a few cytokines. Now it would be an important addition if we can
have some NFkB staining. Literature often has very good IHC stainings but
my student is struggle on it. From some company sites, some antibody works
great. Now I will ask him to try the frozen sections. |
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l********n
发帖数: 260 | 29 最近做wnt pathway， 需要检测中间的samds变化， 请问有没有samds 系列的antibody
kit
因为只要做几次就可以， 不需要分开订购。 谢谢 |
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l***i
发帖数: 36 | 30 We already know there is a protein A have full length and truncated two
isoforms. The full length isoform shares the same sequence as truncated one.
How could I generate antibody that can only recognize truncated isoform in
flow or immune fluorecent staining. Thanks. |
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y****i
发帖数: 60 | 31 最近做用小鼠心脏组织样品做WESTERN BLOTTING，用了好几个beta-tubulin 的抗体都
不给信号或者给unspecific 信号 （不在正确的mw位置）。想请问大家， 你们在做小
鼠心脏组织样品的时候， 都用的哪家的beta-tubulin ANTIBODY ？
万分感谢！！！！ |
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g***y
发帖数: 201 | 32 loading control sampler kit from cell signaling.
all antibodies including beta-tubulin ab are HRP conjugated.
very clean, simple, time saving.
β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) cat# 5346 |
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w*********1
发帖数: 27 | 34 请问各位老师能否帮忙推荐哪个公司有好用的做细胞免疫荧光IF的Rabbit的anti-Flag
或者 Rabbit的anti-HA antibody？ 实验需要必须是Rabbit的， 谢谢各位老师啦。 |
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e***l
发帖数: 33 | 35 发个小广告。
Position: Scientist / Senior Scientist
Area: protein/antibody conjugation
enzyme/dye conjugation
protein immobilization to solid phase
Degree: MS/PhD + 1-4 years experiences
Location: Frederick MD (provide relocation package)
Company: top tier in biotech
Preferred: work experience within biotech industry
Has huge career development potentials.
Has very specific requirement.
So don't feel bad if I tell you it doesn't fit you.
Send your question(s) / resume to e****[email protected] |
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m*1
发帖数: 41 | 36 rt 我用的是Phenomenex Aeris widepore C18 column, 是专门用来分离protein的，
mobile phase 用ACN/H2O 0.1%TFA, 一开始只用两种standard antibody试条件，无奈
尝试了很久都没有分离出来，Gradient slope大了solute就全部一起洗出来，小了peak
就宽到不行，昨天试了一个long gradient用0.1%B/min结果peak宽到几十分钟几乎看不
到...
有没有大侠有过类似经验的给点指导，感激不尽。。。。 |
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t*****m
发帖数: 73 | 37 需要有确切amino acid sequence的Monoclonal antibody，作为mass spec的标准物质
。无所谓biological specificity。
哪里能买到？ |
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l***u
发帖数: 55 | 38 【 以下文字转载自 Biology 讨论区 】
发信人: leofu (nnd，什么时候才能1000), 信区: Biology
标 题: 请推荐做antibody therapy研究的大牛
发信站: BBS 未名空间站 (Fri Oct 9 05:23:34 2009, 美东)
rt |
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m*********3
发帖数: 1425 | 39 我们实验室想买一些monoclonal antibody的药物,最好是CHO cell表达的做研究,现在
市场上各个大药厂这样的上市的药很多,我们没有处方,和几个医生联系过,等了一年也
没有给我们任何样品,我们打算自己买,不知道哪家公司可以买到?谢谢各位 |
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T*******5
发帖数: 2 | 42 A Group Leader/Principle Scientist position in Antibody Drug Discovery is
available at Shanghai Hengrui Pharmaceuticals Co. Ltd.
Responsibility:
- Design and perform research to advance cancer immunotherapy drug discovery
programs. Provide hands-on experimental support.
- Develop and perform in vitro immunoassays including primary lymphocyte
cultures, tumor-lymphocyte co-cultures, etc.
- Establish and run ex vivo tumor models.
- Stay abreast of latest scientific and technology advancements in t... 阅读全帖 |
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S*****a
发帖数: 63 | 46 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
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S*****a
发帖数: 63 | 47 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
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S*****a
发帖数: 63 | 48 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
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S*****a
发帖数: 63 | 49 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
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S*****a
发帖数: 63 | 50 Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
We are seeking an experienced and highly motivated Scientist with strong
hands-on experience in antibody display (phage/yeast) to support discovery
research projects by screening to identify lead candidate therapeutic
antibodies, as well as engineering molecules for improved... 阅读全帖 |
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