p*****m
发帖数: 7030 | 1 我们这一个lab从药厂拿了一大笔钱 做这个circadian rhythm的fish screen。不过我
很奇怪这个东西为啥有药厂关心。。按说要做这个还不如jet lag市场大吧 |
|
p*****m
发帖数: 7030 | 2 那应该去做sleep screen呀， circadian rhythm和sleep还是不太一样的 |
|
a********k
发帖数: 2273 | 3 circadian rhythm？为啥不用fly之类的做？larvae里面这玩意儿做得不清不楚的，没
觉得有啥前途啊 |
|
b****r
发帖数: 17995 | 4 再update一下，给我短信电话之类提问的同修起码也有10多个了，但是基本上都是针对
在遗传，而实际上很多同修根本没有什么遗传经历，可以说我觉得希望可能不是很大。
但是大家完全没有必要局限在遗传方面。我刚刚发现一个简单的找你自己相关
fellowship的方法：
google： "american board of" and PhD
比如第一页就出来个 Sleep Medicine就是PhD可以申请的，如果你现在的生物研究和睡
眠/circadian有关，可能就很有机会。我来美国感觉最深一点的就是，这里的行会保护
很厉害，我迄今知道的，只要有个Board，特别是博士以上水准的，怎么每年也能拿到
近10万甚至10多万，而且一般不用担心失业，因为board的管理者自己也是靠这个board
吃饭的，他们会保证新拿到board的人不至于让他们这些老的失业。最近还听说了有放
疗剂量/定位的fellow，做流式细胞仪的bioanalysis fellow都是PhD qualify的。搞不
好咱们生物PhD在美国其实有一片很广阔的天地等待开采。
&&&&&&&&&&&&&&&&&&&&&&&&&&&... 阅读全帖 |
|
b****r
发帖数: 17995 | 5 再update一下，给我短信电话之类提问的同修起码也有10多个了，但是基本上都是针对
在遗传，而实际上很多同修根本没有什么遗传经历，可以说我觉得希望可能不是很大。
但是大家完全没有必要局限在遗传方面。我刚刚发现一个简单的找你自己相关
fellowship的方法：
google： "american board of" and PhD
比如第一页就出来个 Sleep Medicine就是PhD可以申请的，如果你现在的生物研究和睡
眠/circadian有关，可能就很有机会。我来美国感觉最深一点的就是，这里的行会保护
很厉害，我迄今知道的，只要有个Board，特别是博士以上水准的，怎么每年也能拿到
近10万甚至10多万，而且一般不用担心失业，因为board的管理者自己也是靠这个board
吃饭的，他们会保证新拿到board的人不至于让他们这些老的失业。最近还听说了有放
疗剂量/定位的fellow，做流式细胞仪的bioanalysis fellow都是PhD qualify的。搞不
好咱们生物PhD在美国其实有一片很广阔的天地等待开采。 |
|
b****r
发帖数: 17995 | 6 再update一下，给我短信电话之类提问的同修起码也有10多个了，但是基本上都是针对
在遗传，而实际上很多同修根本没有什么遗传经历，可以说我觉得希望可能不是很大。
但是大家完全没有必要局限在遗传方面。我刚刚发现一个简单的找你自己相关
fellowship的方法：
google： "american board of" and PhD
比如第一页就出来个 Sleep Medicine就是PhD可以申请的，如果你现在的生物研究和睡
眠/circadian有关，可能就很有机会。我来美国感觉最深一点的就是，这里的行会保护
很厉害，我迄今知道的，只要有个Board，特别是博士以上水准的，怎么每年也能拿到
近10万甚至10多万，而且一般不用担心失业，因为board的管理者自己也是靠这个board
吃饭的，他们会保证新拿到board的人不至于让他们这些老的失业。最近还听说了有放
疗剂量/定位的fellow，做流式细胞仪的bioanalysis fellow都是PhD qualify的。搞不
好咱们生物PhD在美国其实有一片很广阔的天地等待开采。 |
|
t*******y
发帖数: 36 | 7 sleep里的circadian clock有意思么？ |
|
r******y
发帖数: 21907 | 8 笑，自己撒网找好了，circadian clock的很多，看paper吧，问那些老板就是啦。估计
你真来了也会心痛的，不过还是祝福你吧，哈哈！ |
|
s******y
发帖数: 28562 | 9 Circadian cycle 和睡眠不是一回事。
虽然植物里（以及大部分生物）都有生物钟周期，但是我觉得它们的这些活动
严格来说不是睡眠。因为在人里面，睡眠和清醒周期的活动不能重叠，也就是
说人不能一边睡眠一边清醒地工作。
但是植物的那些生物钟周期，比方说最经典的光反应和暗反应，其实是可以
同时进行的。而且如果你把一个普通的植物24小时不停的放在光下放上一年，
它就可以24小时不停的进行光反应而不会有什么不良反应。但是如果你把人
放在一个环境里不允许他睡觉，过上一个星期他就死掉了。
and+ |
|
l**********1
发帖数: 5204 | 10 Bingo
plus 病毒proteins ICP0 to remodel viral chromatin Circadian CLOCK
PNAS 2010
web link:
//www.ncbi.nlm.nih.gov/pubmed/20876123
and J Virol. (2011) web link:
//www.ncbi.nlm.nih.gov/pubmed/21734043 |
|
c**l
发帖数: 121 | 11 Sleep. 2005 Apr;28(4):395-409.
Deletion of the mammalian circadian clock gene BMAL1/Mop3 alters baseline
sleep architecture and the response to sleep deprivation.
Laposky A, Easton A, Dugovic C, Walisser J, Bradfield C, Turek F
please send to c*****[email protected]
thanks a lot! |
|
l**********1
发帖数: 5204 | 12 Continue:
Cell cycle/Budding new mechanisms cutting edge approaching:
//adsabs.harvard.edu/abs/2010arXiv1011.2435B
Beersma, D. G. M.; Broer, H. W.; Efstathiou, K.; Gargar, K. A.; Hoveijn, I.
Pacer cell response to periodic Zeitgebers (2011)
Physica D: Nonlinear Phenomena, Volume 240, Issue 19, p. 1516-
cited:
>(5) asymmetry in the observed period
and the phase delay and phase advance components of phase
response curves. Aside from these, we also now have a set
of four directly observable paramet... 阅读全帖 |
|
l**********1
发帖数: 5204 | 13 Continue:
Cell cycle/Budding new mechanisms cutting edge approaching:
//adsabs.harvard.edu/abs/2010arXiv1011.2435B
Beersma, D. G. M.; Broer, H. W.; Efstathiou, K.; Gargar, K. A.; Hoveijn, I.
Pacer cell response to periodic Zeitgebers (2011)
Physica D: Nonlinear Phenomena, Volume 240, Issue 19, p. 1516-
cited:
>(5) asymmetry in the observed period
and the phase delay and phase advance components of phase
response curves. Aside from these, we also now have a set
of four directly observable paramet... 阅读全帖 |
|
l**********1
发帖数: 5204 | 14 Please refer
Harmonics of Circadian Gene Transcription in Mammals
PLoS Genet. 2009 April; 5(4): e1000442.
Published online 2009 April 3
Validation experiments show that 12-hour oscillatory transcripts occur in several other peripheral tissues
as well including heart, kidney, and lungs. These harmonics are lost ex vivo, as well as under restricted
feeding conditions. Taken in sum, these studies illustrate the importance of time sampling with respect to
multiple testing, suggest caution in use of ... 阅读全帖 |
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c********n
发帖数: 67 | 15 Isolation and phenogenetics of a novel circadian rhythm mutant in zebrafish
Identification of a mutation in the Clock1 gene affecting zebrafish circadia
Thanks very much for your kindly help
h***[email protected] |
|
l**********1
发帖数: 5204 | 16 please send to
No.1
Arthur T. Winfree
Biological rhythms and the behavior of populations of coupled oscillators. (
1967)
Journal of Theoretical Biology
Volume 16: 15–42
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/6035757
or DOI link:
//dx.doi.org/10.1016/0022-5193(67)90051-3
No. 2
Elizabeth S. Maywood et al
Circadian timing in health and disease. (2006)
Progress in Brain Research
Volume 153: 253–26
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/16876580
Thank you so much in a... 阅读全帖 |
|
l**********1
发帖数: 5204 | 17 有师爷的 Stanislas Leibler在那里
师叔/伯的Uri Alon 在魏茨曼和哈佛两头站PI位 ps: Harvard is visiting PI.
and
Uri Alon and Naama Barkai both were his PD 15Y ago.
下边的师孙/孙女不成rising star 的天牛 不是得了癌症或其它致命的疾病中途弃权的
就是白犹里的white trash了吧? lol
Please refer:
Mechanisms of noise resistance in genetic oscillatiors
JM. Vilar, HY. Kueh, N. Barkai and S. Leibler
PNAS, 99, 5988-5992 (2002) | PDF
· Robust amplification in adaptive signal transduction networks
N. Barkai, U. Alon and S. Leibler
C.R. Acad. Sci. Ser. IV 2... 阅读全帖 |
|
a**y
发帖数: 464 | 18 如果没有重现性，那不是science。具体说两个人作“同一个”protocol，很有可能一
些细微的
东西没有完全一样， 但是那些细微点可能反而是真正重要的地方。这时候，正确的分
析就很
重要。
另外你举的这几个例子，其实就不是protocol的问题，而是整个community的问题。如果
生物水平上早上和晚上不一样， 如果这个不一样是真实存在的，那么肯定背后有机制
（比如
circadian rhythm）没有研究清楚。而不应该早早下结论。
就算在单个个体上重现性差，统计意义上起码要一致。不然那不是科学，那是掷骰子。 |
|
y***j
发帖数: 11235 | 19 你说的理论我太赞成了，但是实际上不可行。
“很有可能一些细微的东西没有完全一样， 但是那些细微点可能反而是真正重要的地
方。”
对了可能是重要，但是生物细胞是一个系统，太多“真正重要的”的东西了，而很多东
西是不可控的。但是化学只需要分析10个变量，而生物可能需要分析上千个变量。最简
单的T细胞的分化，我就给你说说follow一个protocol时可能产生差别的地方。即使用
相同的培养基，血清不同的批次会有影响。cytokine，就算用了相同的厂家的cytokine
，同样浓度，活性会不同，甚至同一batch，到你手上活性也会有差别，而且不同
cytokine之间还会有相互作用。再加上需要加抗体，有的还需要再day3或者day4换个
cytokine，这里不同细胞可能分化速度不同又可能造成不同。甚至培养瓶甚至
incubator可能都会有影响，我们只能分析重大的几个，然后重新摸下条件。但是有时
候需要摸的条件太多，就造成无法重复。
关于生物水平，我们当然知道可能背后有背后的原因，就简简单单的老鼠早晚杀结果不
同的问题，可能性太多了，circadian rhythm的原因也有很多，可能跟... 阅读全帖 |
|
m**e
发帖数: 857 | 20 恩，he is a neuroscience editor for Nature and co-curator of several groups
at Nature Network.
University of Notre Dame
B.S., B.A.
Double-major in biology and Art History
Mayo Clinic and Graduate School
Ph.D.
Molecular Neuroscience emphasis in the laboratory of Mark McNiven.
Postdoctoral Fellow
Cold Spring Harbor Laboratory
Cold Spring Harbor, NY
03/04 to 09/06
Conducted circuit and plasticity research in the laboratory of Karel Svoboda.
Postdoctoral Fellow
Janelia Farm Research Campus
Ashburn, VA... 阅读全帖 |
|
m**e
发帖数: 857 | 21 Noah Gray is Currently specializing in editing manuscripts on learning &
memory, plasticity, neurodevelopment, axonal guidance & regeneration, sleep
& circadian, social neuroscience, cognitive neural correlates and lots of
other stuff for the journal.
做NEUROCIENCE的，投稿NATURE时点名避开Noah Gray，理由是potential
discrimination.
这样施加压力可行么？ |
|
t****r
发帖数: 66 | 22 MR找工作时坚决不做imaging有关的技术开发，因为觉得没劲，
因为喜欢做science，所以focus在比较小众的cyanbacteria circadian clock，
不然以他在imaging方面的背景和他本人的天分，
如果做single molecule imaging，绝对任何学校随便挑。
他其实一直没下定决心去research university，一直想去teaching school。牛人啊。 |
|
x**4
发帖数: 112 | 23 http://www.ncbi.nlm.nih.gov/pubmed/22147018
Circadian feeding drive of metabolic activity in adipose tissue and not
hyperphagia triggers overweight in mice: is there a role of the pentose-
phosphate pathway?
Endocrinology. 2012 Feb;153(2):690-9. Epub 2011 Dec 6.
Thanks |
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t*******o
发帖数: 424 | 24 至少还有circadian rhythm之类的吧 |
|
c*******0
发帖数: 190 | 25 circadian rhythm主要感知24小时... |
|
c********n
发帖数: 67 | 26 Ultrastructure of rabbit retinal nerve fibre layer--neuro-glial
relationships, myelination, and nerve fibre spectrum
Circadian rhythmicity in neutrophil chemotaxis.
Thanks very much for you kindly help
h***[email protected] |
|
c********n
发帖数: 67 | 27 1, Ultrastructure of rabbit retinal nerve fibre layer--neuro-glial
relationships, myelination, and nerve fibre spectrum
2, Circadian rhythmicity in neutrophil chemotaxis.
3, Concentration of astrocytic filaments at the retinal optic nerve junction
is coincident with the absence of intra-retinal myelination: comparative
and developmental evidence
h***[email protected]
Thanks very much for you kindly help |
|
l**********1
发帖数: 5204 | 28 merci!
plus
PMID 22622569
Nature. 485: 459-64.
by Edgar RS et al.
Peroxiredoxins are conserved markers of circadian rhythms.
http://www.ncbi.nlm.nih.gov/pubmed/22622569
it seems that ancient eubacterail if actin/tubulin/myosin 的总体动态以及相关
的body liquid response factor if hold 生理时钟 then it orgin birth age was
around 2.5 Billion Ys ago when O2/N2 ratio suddendly increaced within the
earth atmosphere...
Should we try testify it by In Vitro method/s? Sunnyday
钟。
dynamics
Geneva |
|
t**l
发帖数: 109 | 29 现在生物医学界的大热点都是哪些，求讨论一下，激发灵感
1. IPS，一直都是焦点。
2. DNA methylation 加epigenetic , 这个月的cell的cover都是DNA甲基化修饰的主题
， epigenetic在cancer,stem cell, germ cell继续热。
3. CRISPR, 最新的那个rudolf jaenisch刚发的那个crispr KO paper,还有 zhang
feng, 感觉如果genome editing除了KI和inducible，会继续火下去。
4. sequencing: 更多的sequence 新方法，还有intron, promoter sequencing, levi
的TERT promoter是不是要引起一阵promoter and intron region sequencing的热潮？
5. 结构：这个就不多说了，那么多结构还没出来了。
6. signaling: hippo, mTOR,
还有就是以前hot过，现在还有点余热的话题
metabolism: IDH
Circadian rhythm： 最近也开... 阅读全帖 |
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h**********n
发帖数: 617 | 30 需要一片paper讲journal club,是science的。
PMID: 24051248
Science. 2013 Sep 19. [Epub ahead of print]
Circadian Clock NAD+ Cycle Drives Mitochondrial Oxidative Metabolism in Mice.
谢谢！
我的邮箱：
h*******[email protected] |
|
m******g
发帖数: 467 | 31 Nature 14.10.30
稍微了解一下：
1. Geneticists tap human knockouts
小知识：
1. Transcriptional interference by antisense RNA is required for circadian
clock function
2. The top 100 papers
3. Teamwork helps yeast to infect
4. Conditional tolerance of temperate phages via transcription-dependent
CRISPR-Cas targeting |
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M**a
发帖数: 4816 | 32 I guess it is about circadian?
can |
|
w*********9
发帖数: 3 | 33 Kast, A., Nishikawa, J., Yabe, T., Nanri, H., and Albert, H. (1988).
Circadian rhythm of liver parameters (cellular structures, mitotic activity,
glycogen and lipids in liver and serum) during three consecutive cycles in
phenobarbitaltreated rats. Chronobiol. Int. 5, 363–385.
Please send to: [email protected]
/* */
Thank you very much |
|
x********e
发帖数: 35261 | 34 circadian clock. 做这个的人很多啊，你肯定是没找对文章。 |
|
T*****e
发帖数: 247 | 35 楼主问得问题过于复杂。我一开始没敢进来回答。
笼统的说，睡眠的研究还很初步，很多东西根本搞不清楚。模式动物的选择也有区别。
我说几个可以研究的参数：
一整天内的睡眠时长。注意：睡午觉也是算的。
每一次进入睡眠的时长。注意：很多失眠患者每天总的睡眠时长并没有变，但是每次睡
的很短，多次入睡。
这两个参数可以用很多的模式动物进行研究，其中部分是由生物钟circadian clock调
控的。
关于人的REM睡眠，我是外行，就不献丑了。
verdelite说了的，我再补充说明几点：
1.和白天黑夜转换有关。是指的光照周期。而光线刺激是最强烈的重设生物钟的信号。
这个不管你是白天睡觉的，比如小鼠。还是晚上睡觉的比如人。又或者中午和晚上睡觉
，黎明和黄昏兴奋的动物比如果蝇。
但是光线并不是唯一的刺激信号，温度，饮食，或者别人把你叫醒，这些都可以让你觉
醒。而觉醒又是一个研究睡眠的参数。
2.睡眠有免疫系统发挥功能的作用。
3.睡眠有清除觉醒时产生的不必要的垃圾的作用。比如在神经系统，楼上有兄弟提出假
设说补充神经递质，这个是已经证明的。主要就是维护稳态。而且觉醒时由经验产生的
脑结构重塑，有一部... 阅读全帖 |
|
v********e
发帖数: 1597 | 36 http://www.nature.com/news/long-sought-biological-compass-disco
In the cells of fruit flies, Chinese scientists say that they have found a
biological compass needle: a rod-shaped complex of proteins that can align
with Earth’s weak magnetic field.
The biocompass — whose constituent proteins exist in related forms in other
species, including humans — could explain a long-standing puzzle: how
animals such as birds and insects sense magnetism. It might also become an
invaluable tool for using m... 阅读全帖 |
|
v********e
发帖数: 1597 | 37 http://www.nature.com/news/long-sought-biological-compass-discovered-1.18803
In the cells of fruit flies, Chinese scientists say that they have found a
biological compass needle: a rod-shaped complex of proteins that can align
with Earth’s weak magnetic field.
The biocompass — whose constituent proteins exist in related forms in other
species, including humans — could explain a long-standing puzzle: how
animals such as birds and insects sense magnetism. It might also become an
invaluable too... 阅读全帖 |
|
u********8
发帖数: 120 | 38 Should I use to help me falling asleep? My normal circadian life style has
been disrupted by doing uw qs of ck. Oftentimes I was awake until early
morning, which really scares me as I will take step2 ck in a month, and
desperately want to be back on right track.
Some pills make people drowsy, but I know many people in the forum had
similar experience. Could you please kindly reccommend one, and I need to
try first, and prove it won't affect me daytime activities, like formal exam.
Thanks in adva |
|
s*****0
发帖数: 357 | 39 提醒你一下血压的circadian波动，如果三次的测量不集中在短时间，time effect是不
能被忽略的。 |
|
S******J
发帖数: 30 | 40 Entry level position:
The University of Pennsylvania, a world-renowned Ivy League research
institution located in Philadelphia, is seeking a Biostatistican in the
Center for Sleep and Circadian Neurobiology. This entry level position is
ideal for a self-motivated candidate looking to gain additional experience
in statistical techniques and programming. The successful candidate will be
exposed to a variety of statistical techniques (classification, re-sampling,
mixed models, nonlinear regression)... 阅读全帖 |
|
z****o
发帖数: 368 | 41 Thx for the case. It seems to be a solitary enhancing mass located near the
amygdala or hypothalamus. Since circadian cycle is controlled by the
hypothalamus("suprachiasmatic nucleus", according to wiki), it is likely
that the pt. is suffering from hypothalamus dysfunction due to the brain
mass. It could also compress the internal capsule or along the pathway,
causing the left side weakness.
Without further info, most possible reason for the mass is brain tumor. At
this age, it is more likel... 阅读全帖 |
|
n***w
发帖数: 2405 | 42 It is logic to reason legally blind people may not have input to SCN which
interacts many other regions of the brain. Such loss may disturb the
endocrine system causing mood/emotion problems. But I don't think there is a
consensus over whether legally blind people have circadian rhythm or not.
I don' know the rewiring process after visual loss at the level of brain
cortex. I learned from many TV shows or movies that blind people have
superior hearing ability. LOL |
|
