s*******e
发帖数: 740 | 1 If you clone a 30 bp DNA into the 5' of the filamentus phage
gene III or VIII, it will express a 10 Aa peptide on the surface
of the phage partical. So if you have a libray of random sequence
30bp DNA, you're able to get a libray of phage with each of
them express a different random 10 Aa peptide on the surface.
That is a 10Aa phage display library.
If you have a ligand and you're able to immoblize it on the coloumn,
you can screen the libray to see which of them bind.Sequence the binding
phage |
|
f*y
发帖数: 876 | 2 T4 and lambda are both dsDNA tailed phages of E.coli. Both of them are being
studied as model phages,T4 as a virulent phage, and lambda as a temperate
phage. Despite of minor sequence similarity, many essential structural
proteins share the same folds, including the major capsid protein, portal
protein, tail tube protein and terminase. So the two phages must have
evolved from a common ancestor. However, because of the different life
styles (virulent/temperate), they have huge differences in the |
|
p*******n
发帖数: 6 | 3 我刚开始做金葡菌的致病因子，要用phage从一种strain上载入一个mutant DNA片段导
入另一个strain。用的phage是transducing phage，据提供者说high frequency of
lysogenization。我用plaques提取lytic phage，然后和recipient混合，incubate 20
分钟以后立即稀释、涂板，结果没有一个带resistant marker的colony长出来。
我的实验方法可能会出什么问题？
这种phage是不是主要进行specialized transduction？收集lysogen然后transduce是
不是可行的方法？
谢谢各位。 |
|
S*****a
发帖数: 63 | 4 【 以下文字转载自 Biology 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Biology
标 题: Yeast Display/Phage Display Scientist position available in Woburn MA
发信站: BBS 未名空间站 (Fri Jul 7 09:38:52 2017, 美东)
We are seeking an experienced and highly motivated Scientist with strong
hands-on
experience in antibody display (phage/yeast) to support discovery research
projects
by screening to identify lead candidate therapeutic antibodies, as well as
engineering
molecules for improved function. The successful candidate wi... 阅读全帖 |
|
p*******n
发帖数: 6 | 5 yes, you got my question.
The mutant comes with an Ab-resistant marker.
I am culture phage with mutant strain, and then collect the phage from
plaques by homogenizing the agar chunks. However, these phage do not produce
Ab resistant colonies on the Ab-selective plates.
I am wondering if only the lysogens can carry the mutant gene with Ab
resistance marker.
Thank you for replying me.
it |
|
b******y
发帖数: 627 | 6 My guess,
Kan slow down the growth a bit. That allows phage to catch up and clear out
your culture.
Can you use TonA knockout strain to do your experiments? if so, you can try
that. (You can get the strain from a number of vendors.) The reason is that
the popular bacteria phage contamination is due to T1 phage. The receptor of
it on E. coli is TonA.
Good luck. |
|
S*****a
发帖数: 63 | 7 We are seeking an experienced and highly motivated Scientist with strong
hands-on
experience in antibody display (phage/yeast) to support discovery research
projects
by screening to identify lead candidate therapeutic antibodies, as well as
engineering
molecules for improved function. The successful candidate will be self-
motivated, enthusiastic, hard-working, detail-oriented, and able to work in
a fast-paced environment.
Responsibilities:
• Drive efforts to isolate, clone, build, and ... 阅读全帖 |
|
C*******e
发帖数: 4348 | 8 我对Phage的知识还停留在最基础的阶段
想问问T4和Lambda到底有多少区别
如果说某个自养细菌的Phage都是类似T4的
那么一个基于Lambda Prophage的同源重组系统在这个自养细菌里能不能work呢？
问题有点弱啊
牛人见谅～！ |
|
A********2
发帖数: 107 | 9 My suggestion is:
1. Test if your recipient strain is phage sensitive
2. Determine how many PFU per ml in your lysate by phage titration assay and
use the appropriate amount of lysate in the transduction. |
|
l**********8
发帖数: 2 | 10 Hi, are there anyone familiar with E coli?
I've screened some mutants and is trying to confirm them by doing site
mutagenesis and spot titration. But, during the process I found my
transformants which were streaked out twice, were contaminated by phage (
precipitates and clear o/n culture without many cells). When I am doing the
o/n culture, I found that if I add Kan, there would be a phage problem, but
if I didn't, there wouldn't. This makes me sooooooo confused and
disappointed. I wonder whet... 阅读全帖 |
|
a****o
发帖数: 1786 | 11 【 以下文字转载自 Returnee 讨论区 】
发信人: penicillin (penicillin), 信区: Returnee
标 题: 北京外企招聘 Scientist/Senior Scientist, Antibody Phage Display
发信站: BBS 未名空间站 (Tue Mar 16 22:53:03 2010, 美东)
Responsibility:
-Lead effort on antibody discovery/generation via phage display selection
process
-contribute to strategic and scientific activities related to the discovery
and design of recombinant antibody
-interact with discovery scientists and develop effective inter-departmental
and cross-functional relationships, |
|
p********n
发帖数: 26 | 12 Responsibility:
-Lead effort on antibody discovery/generation via phage display selection
process
-contribute to strategic and scientific activities related to the discovery
and design of recombinant antibody
-interact with discovery scientists and develop effective inter-departmental
and cross-functional relationships, including international interactions
-identify, evaluate and manage potential external collaborators and research
opportunities
-expand awareness of novel technology development |
|
H**********J
发帖数: 351 | 13 如题。
国内antibody蛮多人做；但是Phage Display不到30人。。。国外如何？求解~
国内做的最好的是被罗氏买来的Glycart；有职位Open,但是木有人。。。。 |
|
H**********J
发帖数: 351 | 14 做antibody的同学，做过Phage Display的，博士或者博后有愿意回国的联系我吧：
j****[email protected]; |
|
H**********J
发帖数: 351 | 15 这个职位没有，呵呵。不用很资深~只要有Phage Display经验就行~ |
|
A********2
发帖数: 107 | 16 If I understood your question correctly, you have inserted a Ab resistance
gene (linked to the mutation) into the mutant chr and were trying to move it
into wild type strain. Why not just make a phage lysate of your mutant
strain and then transduce the wild type strain. |
|
l**********8
发帖数: 2 | 17 Thank you so much!
My today's experiment also shows that after adding Kan, my bacteria got a
phage problem probably due to the reason you mentioned above.
out
try
that
of |
|
S*****a
发帖数: 63 | 18 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
|
S*****a
发帖数: 63 | 19 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
|
S*****a
发帖数: 63 | 20 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
|
S*****a
发帖数: 63 | 21 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Somalia (Anti-Pirates), 信区: Pharmaceutical
标 题: Biologics Discovery and Development Positions Available at Abpro Labs in Woburn, MA
关键字: Biologics,Discovery,Development,Antibody,Computational
发信站: BBS 未名空间站 (Wed May 31 20:28:52 2017, 美东)
Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
... 阅读全帖 |
|
S*****a
发帖数: 63 | 22 Multiple Biologics Discovery and Development Positions Available at Abpro
Labs in Woburn, MA
www.abpro-labs.com
To apply please send resume to [email protected]
1. Scientist/Senior Scientist, Antibody Display Technology
We are seeking an experienced and highly motivated Scientist with strong
hands-on experience in antibody display (phage/yeast) to support discovery
research projects by screening to identify lead candidate therapeutic
antibodies, as well as engineering molecules for improved... 阅读全帖 |
|
k***r
发帖数: 100 | 23 One possible way is using Bac. Phage. Since it can random pick up Bac. genome. So
you get a lirary of phage with random insert, use it to do complement test,
see which one will recover the lost function. But the problem is once you get
the phage that has right insert DNA, how do you get it out? Or you have to do
Sanger's seq. through the phage genome (which is impossible at 50s'):( |
|
T**********t
发帖数: 1604 | 24 看起来很像phage contamination。
我碰到过类似的情况，不过不是外来的phage contamination，更像是大肠杆菌里自带
的phage进入了lytic cycle。我那次是因为培养箱温度显示出错，实际温度过高造成的
，后来把温度调低了就好了。你检查一下你的培养箱温度看看。 |
|
w******e
发帖数: 1187 | 25 http://www.nature.com/nbt/journal/v29/n6/full/nbt.1856.html
phage display是好东西。。。and又见next-gen seq in directed
evolution。。。well只做一个round的话不知还该不该叫evolution lol
不过这篇paper的pos control够单薄的。
Immune responses targeting self-proteins (autoantigens) can lead to a
variety
of autoimmune diseases. Identification of these antigens is important for
both diagnostic and therapeutic reasons. However, current approaches to
characterize autoantigens have, in most cases, met only with limited success
. Here we pres... 阅读全帖 |
|
l**********1
发帖数: 5204 | 26 phage group 的
卢瑞亚-德尔布吕克实验
早就指出了 bac resistant to phage 进化是自然环境选择的随机概率 事件 而不是
适应性变异事件
不是 适应phage attack bac 者继续后代 概率大
而是randomly gained higher fittness bac 继续后代 概率大
please refer PPT file:
//www.nicholls.edu/biol-ds/biol370/Lectures/Genetic%20Variation%202.pdf
卢瑞亚-德尔布吕克实验指生物学家萨尔瓦多·卢瑞亚与马克斯·德尔布吕克在1943年
所做的一些生物学实验，这些实验证实细菌对噬菌体的抵抗能力主要来自于自然选择而
非适应性变异
paper link:
/www.genetics.org/content/136/3/1209.full.pdf
or
//en.wikipedia.org/wiki/Luria–Delbrück_experiment |
|
b******y
发帖数: 627 | 27 When we had similar problem 10+ years ago, we determined that the phage is
t1 using pcr. We knew from literature that t1 phage gets into cells via tona
, a copper or ion transporter, or something alike. There are tona knockout
strain commercially availabe bl21de3 and dh5alpha, which are called
something like bl21de3 T1r and dh5alpha T1r. T1r means T1 phage resistant.
That really solved the problem once and for all.
Good luck! |
|
w*****3
发帖数: 1582 | 28 phage display 技术早就有了，但是关键的专利掌握在少数几个公司手里，很难开展
commercial的work， 最近有些专利到期了，新的工作才开展起来。 phage display 筛
的好处是可以用high throughput 筛+ next generation sequencing， 效率高，是人
源性抗体，关键的步奏是phage display 的 library 的建立， |
|
l****y
发帖数: 398 | 29 就是筛出来的东东immunogenicity有点高，
[在 wangyi3 (wang3yi) 的大作中提到：]
:phage display 技术早就有了，但是关键的专利掌握在少数几个公司手里，很难开展
:commercial的work， 最近有些专利到期了，新的工作才开展起来。 phage display
筛的好处是可以用high throughput 筛+ next generation sequencing， 效率高，是人
:源性抗体，关键的步奏是phage display 的 library 的建立， |
|
w*********g
发帖数: 30882 | 30 笔者对此发表以下看法：
（1）卫生部2007年颁布实施《新资源食品管理办法》有意取消了2002年颁布实施
的《转基因食品卫生管理办法》“第五条 转基因食品的食用安全性和营养质量不得低
于对应的原有食品”这个条款，是退步，而不是进步！这种删除的实质是，进一步顺从
邪恶孟山都为首跨国转基因产业的私利与压力，置中国人民持续安全、健康、生存与繁
衍于不顾！
（2）卫生部2007年颁布实施《新资源食品管理办法》有意取消了2002年颁布实施
的《转基因食品卫生管理办法》“第五条”，但是卫生部《新资源食品管理办法》“第
三条”规定：
第三条 新资源食品应当符合《食品卫生法》及有关法规、规章、标准的规定，对
人体不得产生任何急性、亚急性、慢性或其他潜在性健康危害。
依据该第三条规定，在对于抗草甘膦转基因大豆加工的转基因大豆油，检测出超过
俄罗斯对大豆油草甘膦残留量允许标准与中国卫生部对棉籽油草甘膦残留最高限量2.9-
6倍的草甘膦残留量，已经证实对人类健康，特别对青少年、儿童、婴儿与孕妇及其胎
儿，造成极其严重危害情况下，（1）对人类健康不是形成 “潜在性健康危害”而是... 阅读全帖 |
|
h****z
发帖数: 1996 | 31 不错啊。我也发现玩Jax的人多了起来。关于装备选择，下面是我的一点个人看法：
Phage小冰锤 vs Cutlass弯刀
两者差不多，先出哪个差别不太大。如果gank多、顺风，可以先出phage，多一点HP也
能帮助多抗一下塔。逆风的话先出Cutlass，可以安全地farm lane/jungle，也可以用
来逃跑。
Gunblade枪刀 vs BOTRK陨落
Gunblade提供的AP+AD更好，可惜没有提供攻速，BOTRK追人、逃跑、1v1效果
更好。如果喜欢出Gunblade，可以带9个attack speed marks加起来有15%的攻速，或者
出个Wits End。如果对面一直推线给的压力很大，我建议出BOTRK然后推线，BOTRK是
1v1分推神器，很少有英
雄可以1v1 Jax。
Randuin vs Warmog
Randuin提供的utility太好了，除非对面mid和top都是AP，然后对面ADC很弱（比如中
前期Tristana），我才会考虑Warmog。像你的第一张图里面对面几乎是全AD，先出
Randuin效果会比较好。 |
|
s*****a
发帖数: 450 | 32 【 以下文字转载自 ChuanYu 讨论区 】
发信人: phage (phage), 信区: ChuanYu
标 题: 嫁人还是要嫁成都小伙子
发信站: BBS 未名空间站 (Sat Dec 6 19:09:32 2008)
突然开窍了，发现还是成都帅哥多啊。当年我们高中，从高一到高三，简直帅哥多惨了
。皮肤好，身材好，个性好，真的是上得厅堂，下得厨房。我现在还记得当初高三还有
同年级四班一个男生，简直就是漫画中走出来的--还是活的。
后来到了大学，出国；我早已经是心如古井，不起微澜勒。北方的轮廓太粗，上海的太
伪娘，西方人只可远观，不可亵玩。
肇了三，下手晚了。 |
|
d***e
发帖数: 243 | 33 我来补充一下,不是查google的,是三年前在北医帮导师写教材时我的一些理解.
Transfect一般是指各种artificial的手段给动物细胞内导入外源的基因片段.
Transduction ,conjugation涉及的是细菌间的基因转移,不关真核细胞的事.
1. 转化(Transformation).受体菌直接摄取供体菌游离DNA片段,转化的DNA片段可以是细
菌溶解 释放,也可以是人工提取. 摄取能力称为感受态 competence.应用吗,可以讲是最
早证实了DNA是遗传物质的Griffith的肺炎球菌实验.
2.转导 (Transduction). 温和噬菌体介导的遗传物质从供体菌到受体菌的转移.这里强调
的是temperate phage, 不是指溶原性噬菌体lysogenic phage.作用是细菌自发获得一些
新的protein,eg. toxin or antigen.
3.接合(Conjugation). 是plasmids介导的细胞间接触,是供体菌遗传物质进入受体菌.供
体菌遗传物质不一定就非是供体菌的gene,也可能只是供体菌的质粒transfer到rece |
|
d********r
发帖数: 303 | 34 Mycobacteriaphage (EM) image
alpha3 bacteriaphage (structure determined by X-ray and cryo-EM)
Look at the figure above, the tail of the phage is kind of fibular protein,
not globular as most proteins do.
If you look at the genome of bacteriaphage, you will find most recombination
events are outside the gene boundary. The explaination is that any
recombination within a gene will result in defective protein. This obviously
will lead to the elimination of the phages that carries this recomination
e |
|
K*F
发帖数: 120 | 35 简而言之吧，我在做大肠杆菌的P1 transduction。我要把donor菌（ArgE::Tn10(Tet_R
)）转化到
recipient 菌(Tet_sensitive).
做P1 transduction好多次做不出来，一个colony都长不出来。然后做了一个P1 lysate
的titer，
发现稀释到10E-6之后，噬菌斑直径仍然有2cm大小。我该怎么办？
疑问：
（1) recipient菌本身已经有一个Tn10（Tet_sensitive, Streptomycin_resistent）
。奇怪吧，
第一次听说Tn10是Strep抗性的，一般都是Tet抗性的啊。问题是：如果recipient菌已
经有一个
Tn10，我还能通过抗生素筛选的方式转化入另外一个Tn10么？
（2）我的P1 phage lysate 是不是太浓了？我是不是应该做很多个phage lysate的梯
度呢？
不胜感激。 |
|
T**********t
发帖数: 1604 | 36 这个culture开始长得好像正常，虽然慢一点。可是过了大概十三四个小时之后culture
就开始变清，然后出现这种短线状悬浮物。我怀疑是phage contamination，可是不能
确定，有没有人有经验的？如果真是phage就惨了。。。 |
|
T**********t
发帖数: 1604 | 37 E.coli的BL21(DE3)pLysS strain。带的质粒是个小质粒，表达的蛋白不大，只有7kD，
没有细胞毒性。用的培养基是LB+M9 salts，抗生素是Amp+Chloramphenicol。关键是以
前摇菌（同一个strain）都没有这个问题，最近屡屡出现，我不知道是哪里
contaminate了，如果是phage的话，就恐慌了，据说很难清除phage。 |
|
H****s
发帖数: 301 | 38 Usually people achieve this by phage/yeast antibody display technology. You
need to have a very good antibody library and yeast/phage library panning
capability. Once you have a pool of selected binders, you can screen them by
ELISA. In your case, two ELISA can be performed in parallel, one coated
with your target protein, the other coated with negative target protein. The
ELISA signal will give you a rough idea about target specificity. If you
have access to flow cytometry, combining flow sorti... 阅读全帖 |
|
n*********m
发帖数: 38 | 39 Look his publication
Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights
from structural and biochemical studies on human RPE.
Liang W, Ouyang S, Shaw N, Joachimiak A, Zhang R, Liu ZJ.
FASEB J. 2011 Feb;25(2):497-504. Epub 2010 Oct 5.
PMID:
20923965
[PubMed - indexed for MEDLINE]
Related citations
2.
Structural basis for the inhibition of human 5,10-methenyltetrahydrofolate
synthetase by N10-substituted folate analogues.
Wu D, Li Y, Song G, Cheng C, Zhang R, Joac... 阅读全帖 |
|
t*******n
发帖数: 446 | 40 我们也想过是phage污染，不过因为还能长起来，所以就排除了。大肠杆菌自己带的
phage污染是什么机理呢？如何解决？
我们有两个培养箱，应该不至于都坏掉的。 |
|
w******e
发帖数: 1187 | 41 让我对droplet/IVC的饭度大减呵呵。跟phage比也有definitive的优势——
毕竟是HT screening而不是selection。我小见识浅薄一惊一乍大家别见怪呵呵。
ms就wittrup一枝独秀？国内有phage/yeast display的牛人吗？
内行指点一下？ |
|
i*****g
发帖数: 11893 | 42 很有意思，看见这么多人在说这个想法。
这个想法我在2005年1月之前，换实验室时，和PI说了，他听不懂这张PPT意味什么。
后来我又换了实验室，2007年（2008？）某天新老板和我听完某个不相关的seminar，
和我说起这个，他才开口说了2句，我就知道他想说什么，
他的反应是，i have not finished my briefing yet, you really understand my
point? 于是就和他说，of course i know what you want to do,然后讨论了半天，原
来他的朋友有这个想法 etc etc，后来我又做了一些Homework，还是决定放弃。这个很
不好做，估计是个死途。
这个技术最大意义是：能找到locus specific DNA-protein complexes。目前的通行做
法是，已知某个protein, 你用CHIP，证明在某个Locus上面，然后开始making story，
编剧。但其实，那个Locus上面可能有几十种特殊蛋白在调控！！！
2004年底，我的想法来源于推广TAP+MS，我已知 TAP+MS... 阅读全帖 |
|
r*****t
发帖数: 4793 | 43 谢谢大家。如果是phage， 有什么办法去掉？用这个MAX Efficiency® DH5α&#
8482; T1 Phage Resistant Competent Cells做转化？
另外摇菌的时间不到20小时，菌落应该不是卫星菌， |
|
C*******e
发帖数: 4348 | 44 SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer
assembly
Nature 2012 doi:10.1038/nature11155
里面提到的这个nanobody就是camelid single chain antibody （heavy chain)
这个方法最初发表于去年
Nanobody stabilization of G protein-coupled receptor conformational states
Current Opinion in Structural Biology
Volume 21, Issue 4, August 2011, Pages 567–572
他们的做法是把目标蛋白打到驼羊身上
从血液里分离淋巴细胞，然后提RNA，做RT，得到antibody mRNA的pool
然后克隆到phage display vector里面
做粗筛
筛出只跟非变性目标蛋白结合的heavy chain antibody
然后跟目标蛋白共表达，做结晶，解结构
我的问... 阅读全帖 |
|
a**y
发帖数: 464 | 45 Several postdoc positions are available in the group of Dr. Huiwang Ai at
the University of California, Riverside http://ailab.ucr.edu. The expertise of our lab is on protein engineering and fluorescent redox probes. Recently, our lab has explored new areas, including the biology and toxicology of oxidative stress, imaging of brain activities, and protein/peptide enzyme inhibitors.
We are looking for postdoctoral fellows with the following
expertise:
1. LC-MS/MS based proteomi... 阅读全帖 |
|
j****n
发帖数: 3370 | 46 这玩意和phage display比如何？感觉phage用的比较多 |
|
f***x
发帖数: 1524 | 47 hybridomas筛的是🐭源抗体，需要人源化。前期需要免疫动物。immune
response 是个问题。优点是有in Vivo quality control,一般不再需要affinity
Maturation and solubility/stability optimization.
相比较，display 可以从人工库筛，库可以很大(10e7-10e11)，用时短，不用考虑免疫
问题。但是往往需要affinity maturation.
Phage display 筛fragment.
yeast display可以 筛full length antibody.
方法上，phage display 简单。主要是Elisa and affinity binding/washing.
yeast display 用到FACS,可以对筛选有很好的控制，同时筛Antibody expression/
stability 和antibody-antigen binding. |
|
z*t
发帖数: 863 | 48 受教了！您怎么看alpaca里搞得nanobody?
：hybridomas筛的是🐭源抗体，需要人源化。前期需要免疫动物。immune
：response 是个问题。优点是有in Vivo quality control,一般不再需要affinity
：Maturation and solubility/stability optimization.
：相比较，display 可以从人工库筛，库可以很大(10e7-10e11)，用时短，不用考虑免
疫问题。但是往往需要affinity maturation.
：Phage display 筛fragment.
：yeast display可以 筛full length antibody.
：方法上，phage display 简单。主要是Elisa and affinity binding/washing.
：yeast display 用到FACS,可以对筛选有很好的控制，同时筛Antibody expression/
：stability 和antibody-antigen binding. |
|
发帖数: 1 | 49 各位前辈好，
本人今年博士第六年，正面临着毕业找工作。博士期间的工作主要有两个方面，1.
using phage display library (peptide) to screen and identify tumor specific
antigen ligands.
2. development of novel siRNA delivery system.
掌握的技术有
1. Screening and identification of targeting ligands using phage display
2. Culture and propagation of transformed cell lines and primary cell lines(
HUVEC) 3. Cellular biology related techniques: MTT and SRB assay, siRNA
transfection, ELISA, Western blot, RT-PCR, Real time PCR, flow cytometry,
Gene cloning... 阅读全帖 |
|
C********g
发帖数: 9656 | 50 【 以下文字转载自 Joke 讨论区 】
发信人: skater (没有最yy只有更yy), 信区: Joke
标 题: zz末世拍案惊奇 第一章：北戴河督抚朝圣驾，小茶馆草民议时局
发信站: BBS 未名空间站 (Mon Aug 8 09:33:56 2011, 美东)
发信人: phage (Red Devils), 信区: Joke
标 题: zz末世拍案惊奇 第一章：北戴河督抚朝圣驾，小茶馆草民议时局
发信站: 水木社区 (Mon Aug 8 18:55:12 2011), 站内
发信人: mimi007 (xx), 信区: NewExpress
标 题: zz末世拍案惊奇 第一章：北戴河督抚朝圣驾，小茶馆草民议时局
发信站: 水木社区 (Mon Aug 8 18:53:06 2011), 站内
一入了七月，天气就开始闷热起来，日头不见得怎么毒，却好似下了火一般，压的人喘
不过气来，也不知怎地，这满城的知了开始骚动起来，又是喊又是叫，却不知朝廷的老
爷们早就不耐烦听了，接连几道命令下来，这满城尽是六扇门的捕快粘知了，一夜之间
，除了几个悍不畏死的还在呱噪，这偌大的城... 阅读全帖 |
|
