C******n
发帖数: 941 | 1 the more harsh your solution, the more components will disolve, of course the
more you will see on the gel.
If you want to disolve all the pellet, try the most "harsh" solution you can
imagine, like 5%-Triton-2%SDS-8M urea..... | C******n
发帖数: 941 | 2
interfere
~~~~~~~~~~~~~~~~~~~~~~~~ this is not harsh to
disolve
all proteins, like some membrane proteins, they are really hard to disolve.
High concentration of TritonX-100 will affect SDS-PAGE, you can try 8M urea,
or you can even add 8M urea when casting the SDS-Gel, search methods with key
words like" disolve membrane proteins", "membrane protein SDS-PAGE", you'll
get more informative stuff.
Good luck
can
buffer |
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