j*******8
发帖数: 933 | 1 Ru-Ti.
I'm testing protein interaction in the post-synaptic density (PSD) fraction
of brain tissue. Usually, we use 1% SDS to dissolve the PSD pellet. However,
we're now planning to test protein interaction with IP. Can anyone tell if
1% SDS interupt protein interaction? what about 0.1%?
Thanks! | a*****n
发帖数: 2835 | 2 buhao
还是用别的detergent吧
1％ triton x－100
fraction
However,
if
【在 j*******8 的大作中提到】
: Ru-Ti.
: I'm testing protein interaction in the post-synaptic density (PSD) fraction
: of brain tissue. Usually, we use 1% SDS to dissolve the PSD pellet. However,
: we're now planning to test protein interaction with IP. Can anyone tell if
: 1% SDS interupt protein interaction? what about 0.1%?
: Thanks!
| j*******8
发帖数: 933 | 3 Thank you for your reply!
But we can't use 1% triton. because the PSD fraction is not sollulable in 1%
triton.
anyway, it's good to know that 1% SDS is not goog for IP. thank you! |
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