g***1
发帖数: 24 | 1 Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences! |
y******8
发帖数: 1764 | 2 4-5% resolving gel and transfer buffer without methanol. |
v***a
发帖数: 1242 | 3 without methanol是什么原理啊？
【在 y******8 的大作中提到】
: 4-5% resolving gel and transfer buffer without methanol.
|
i********e
发帖数: 50 | 4 I use 6% separation gel and transfer without methanol for one hour at 100 V
. My protein is 550 KDa. |
s******r
发帖数: 2876 | 5 invirogen 有 Tis-acetate gel，据广告适合大蛋白。
Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences!
【在 g***1 的大作中提到】
: Does anyone have experiences in western blot handling high molecular weight
: (250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
: sharing your experiences!
|
i*********t
发帖数: 78 | 6 add SDS in trnasfer buffer. |
p********t
发帖数: 411 | 7 Are you labeling Ryanoding Receptor?
V
【在 i********e 的大作中提到】
: I use 6% separation gel and transfer without methanol for one hour at 100 V
: . My protein is 550 KDa.
|
