c*****u
发帖数: 439 | |
c*****u
发帖数: 439 | 2 streptavidin是多聚物 实在没有办法用。
提前谢谢提供线索的各位啊 |
s********x
发帖数: 472 | 3 如果是要化学label的话有很多类似click chemistry的方法。
如果只是结合，好像halotag有小分子可以bind。但是都不普及，不知道work的怎么样。 |
c*****u
发帖数: 439 | 4 非常感谢 已经再化学版 提问了
样。
【在 s********x 的大作中提到】
: 如果是要化学label的话有很多类似click chemistry的方法。
: 如果只是结合，好像halotag有小分子可以bind。但是都不普及，不知道work的怎么样。
|
A******y
发帖数: 2041 | 5 Not possible unless you form a specific covalent bond, which will depend on
your protein. There are some very specific covalent inhibitors. |
M******g
发帖数: 152 | 6 you can try SNAP tag https://www.neb.com/tools-and-resources/feature-
articles/snap-tag-technologies-novel-tools-to-study-protein-function) with
BG substrates https://www.neb.com/products/s9151-bg-gla-nhs or https://www.
neb.com/products/s9153-bg-maleimide).
BG is a small molecule and it will form covalent bond with SNAP protein.
BG can be modified with GLA-NHS group (primary amine reactive) or maleimide
(-SH group reactive). So BG-GLA-NHS or BG-maleimide can be attach to a
protein via primary amine or SH group, and SNAP will then react with BG to
form a covalent bond.
Hope this helps. |
c*****u
发帖数: 439 | 7 非常感谢
on
【在 A******y 的大作中提到】
: Not possible unless you form a specific covalent bond, which will depend on
: your protein. There are some very specific covalent inhibitors.
|
c*****u
发帖数: 439 | 8 嗯 感谢这么详细的答复
www.
maleimide
【在 M******g 的大作中提到】
: you can try SNAP tag https://www.neb.com/tools-and-resources/feature-
: articles/snap-tag-technologies-novel-tools-to-study-protein-function) with
: BG substrates https://www.neb.com/products/s9151-bg-gla-nhs or https://www.
: neb.com/products/s9153-bg-maleimide).
: BG is a small molecule and it will form covalent bond with SNAP protein.
: BG can be modified with GLA-NHS group (primary amine reactive) or maleimide
: (-SH group reactive). So BG-GLA-NHS or BG-maleimide can be attach to a
: protein via primary amine or SH group, and SNAP will then react with BG to
: form a covalent bond.
: Hope this helps.
|
f**u
发帖数: 346 | 9 印象里好象有monomeric streptavidin吧，也许对你来说可以用？ |
M******g
发帖数: 152 | 10 kd for monomeric streptavidin is high, 10e-9, as compared to the kd of
tetrameric streptavidin, < 10e-12 |
