b******n
发帖数: 4225 | 1 RP-HPLC
流动相是a gradient of 0–35% CH3CN (with 0.1% TFA) in water (with 0.1% TFA)
目的是检测medium中的我们感兴趣的物质被细菌利用的情况
我查了一下，MEM-alpha中磷酸二氢钠的浓度大概1 mM
这个medium我直接拿过来酸化之后用0.22um的filter过滤
能否直接上HPLC的柱子[Vydac C18 (22 x 250 mm) column]
会不会堵上？
请有经验的人，尤其是做过细胞培养基分析的人指点一下
谢谢啦 |
m**n
发帖数: 206 | 2 What is the pore size of the column? 300A? much smaller than filter pores |
m**n
发帖数: 206 | 3 also 5 um particles give intra particle pore size around 0.75 um, not much
larger than filter pores, could get clogged. |
y**********t
发帖数: 37 | 4 From my personal experience, as long as samples get filtered by .22 um, it
should be fine. My sample substrates are even worse than yours.
)
【在 b******n 的大作中提到】
: RP-HPLC
: 流动相是a gradient of 0–35% CH3CN (with 0.1% TFA) in water (with 0.1% TFA)
: 目的是检测medium中的我们感兴趣的物质被细菌利用的情况
: 我查了一下，MEM-alpha中磷酸二氢钠的浓度大概1 mM
: 这个medium我直接拿过来酸化之后用0.22um的filter过滤
: 能否直接上HPLC的柱子[Vydac C18 (22 x 250 mm) column]
: 会不会堵上？
: 请有经验的人，尤其是做过细胞培养基分析的人指点一下
: 谢谢啦
|
b******n
发帖数: 4225 | 5 Thank you very much
【在 y**********t 的大作中提到】
: From my personal experience, as long as samples get filtered by .22 um, it
: should be fine. My sample substrates are even worse than yours.
:
: )
|
