T**********e
发帖数: 29576 | 1 Maurice Wilkins把Franklin的DNA X-RAY 背着她给Watson 和 Crick展示,成全这两位
推导出DNA双螺旋结构发表在NATURE, 最后这三个老哥得了诺贝尔奖. 而Franklin得癌
症死掉.
Celebrating Rosalind Franklin: a perfect example of single-minded devotion
to research
Today's Google Doodle honours pioneering British biophysicist and x-ray
crystallographer, Rosalind Franklin
Today is the 93rd anniversary of the birth of British biophysicist Rosalind
Franklin, who was a pioneering x-ray crystallographer who is being honoured
today with a special Google Doodle.
N... 阅读全帖 |
|
m********5
发帖数: 17667 | 2 作为学物理学的，我认为答案是possible, but unlikely
你相当于问，如果我合成一堆塑料，陶瓷，加上一堆金属，堆在一起就能形成发动机么？
又相当于问，如果我有一堆元器件，他们随机堆在一起就能形成计算机么？
细胞并不是简单的生物大分子集合，细胞是一个复杂的分子机器，其中大量生物分子并
不是像溶液中一样自由反应的，他们被精密得组合在不同的时空中，在特定的地点特定
的时间发挥特定的作用; 这部分子机器精致到可以自己复制自己。
这样的机器随机形成是非常非常小概率的事件。你可以用下面这个例子想像一下它有多
困难：
你用一种计算机语言，C++,Java,python,等，把里面的有效字符，函数当成一个元素，
然后让他们随机组合在一起，企图自动产生一个可以在操作系统中自己复制自己的程序
。这个几率是相当低的。何况物理世界比这个复杂得多。
The bases of the traditionally biology, molecular biology, are in vain. The
real biology is synthetic biology. The real impor... 阅读全帖 |
|
u***r
发帖数: 4825 | 3 http://www.nytimes.com/2015/01/29/us/charles-h-townes-physicist
Charles H. Townes, a visionary physicist whose research led to the
development of the laser, making it possible to play CDs, scan prices at the
supermarket, measure time precisely, survey planets and galaxies and even
witness the birth of stars, died on Tuesday in Oakland, Calif. He was 99.
His daughter Linda Rosenwein confirmed his death.
In 1964, Dr. Townes and two Russians shared the Nobel Prize in Physics for
their work on micro... 阅读全帖 |
|
b********n
发帖数: 38600 | 4 "The form and dynamics of every living organism on this planet is controlled
by a single molecule of DNA. Recent experiments suggest that size alone is
not a bar to quantum behaviour. A group based in Vienna have recently fired
fullerene molecules through the double slit experiment and demonstrated that
these particles have no problem in sailing through both slits
simultaneously. And fullerene is big - 60 carbon atoms in a cage-like
structure, the famous 'buckyball' molecule - with a diameter si... 阅读全帖 |
|
C*I
发帖数: 4736 | 5 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 6 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 7 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
|
P******l
发帖数: 1648 | 8
That's a great question!
It's leaking all the time but very slow. (The current rate of loss is about
three kilograms (3 kg) of hydrogen and 50 grams (50 g) of helium per second.
according to the answer in the link below)
If space is a vacuum then why doesn't it suck in all the air from Earth's
atmosphere?
https://www.quora.com/If-space-is-a-vacuum-then-why-doesnt-it-suck-in-all-
the-air-from-Earths-atmosphere
Well the great vacuum does "pull" earth atmosphere if thats the correct way
to say it.... 阅读全帖 |
|
o****u
发帖数: 1299 | 9 One freind of mine who just became a Assistant Professor at ETH (Swiss
Federal Institute of Technology) Zurich is looking for PhD
students/post-docs for his research group in the field of attosecond
science. He is very young (<30), very smart (the most intellegient
yound sciendtest I've ever met) and employees at ETH are very well
paid (~40-50K USD for PhD students and 70-100K for post-docs). See
below for details:
PhD and postdoc positions in attosecond molecular science are
available at the La... 阅读全帖 |
|
s******y
发帖数: 28562 | 10 现在红遍天下的光遗传学，大家（包括我）都认为是Stanford的Deisseroth创造的。但
是昨天我不小心看到了一篇新闻文章，才惊讶的发现其实最早做出来的，有可能是
Wayne State University的潘卓华。虽然他的投稿时间和Deisseroth的类似，但是因为
他是一个没有什么名气的学者，而且他的文章包装得不对，所以他的文章被百般耽误，
最后比Deisseroth的晚了一年才发表。
在2004年底潘就做出了和Deisseroth几乎一样的文章，除了用的细胞略微不同：他做的
神经细胞是从视网膜分离的，而Deisseroth 做的神经细胞是从大脑分离的。然后他把
文章陆续投向Nature, Nature Neuroscience,Journal of Neuroscience，却统统遭到
了拒稿。然后大半年之后，也就是2005年8月份，Deisseroth 的文章发表在Nature
Neuroscience上。潘得知之后，心都凉了。
有意思的是潘卓华发现自己的文章被人scoop之后写信问 Nature Neuroscience为什么
两篇几乎一模一样的文章，他们接受了De... 阅读全帖 |
|
l**********r
发帖数: 240 | 11 It is the major constituent of fat and oil, formed by esterification of
glycerol, a condensation reaction between the hydroxyl group of glycerol
molecule and the carboxyl group of a fatty acid molecule(脂肪酸). When all
three hydroxyl groups of a glycerol molecule are esterified, it becomes a
triglyceride.
在我们体内，它是脂肪的主成份。通常意义上讲的饱和或不饱和脂肪，指的是脂肪酸的饱
和程度，把甘油部份给忽略了。
like
amounts. |
|
d*****r
发帖数: 2583 | 12 【 以下文字转载自 Postdoc 讨论区 】
发信人: demoner (New York State of Mind), 信区: Postdoc
标 题: new postdoc/PhD RA position available at Univ of Michigan.
发信站: BBS 未名空间站 (Tue Jun 26 15:50:19 2012, 美东)
帮一个好朋友贴一个招人广告，这个方向在micro/nano-system和stem cell
proteomics结合的领域，现在交叉学科里面最活跃的方向之一。有两个职位，一个PHD
RA position, 一个postdoc fellowship，在University of Michigan的Mechanical
Engineering Dept，我都贴到下面了。如果有兴趣的请直接跟他联系。
http://me.engin.umich.edu/ibbl/employment.htm
New Graduate Research Assistant Opening in Nanofluidics and
Single-... 阅读全帖 |
|
r********h
发帖数: 5638 | 13 Reverse osmosis (RO) is a water purification technology that uses a
semipermeable membrane. This membrane technology is not properly a
filtration method. In reverse osmosis, an applied pressure is used to
overcome osmotic pressure, a colligative property, that is driven by
chemical potential, a thermodynamic parameter. Reverse osmosis can remove
many types of molecules and ions from solutions, and is used in both
industrial processes and producing potable water. The result is the solute
is retai... 阅读全帖 |
|
g****y
发帖数: 26 | 14 We are seeking highly motivated individuals for postdoctoral positions in
the Structural Biology Program of the Sloan-Kettering Institute, Memorial
Sloan-Kettering Cancer Center, NYC, USA.
The lab develops cutting-edge techniques (Nature,doi:10.1038/ nature09163 (
2010)) aimed at gaining a mechanistic understanding of fundamental
biological processes. Major emphasis is placed on development of novel
single-molecule detection and manipulation instrumentation that pushes the
envelop in sensitivity... 阅读全帖 |
|
d*****r
发帖数: 2583 | 15 帮一个好朋友贴一个招人广告，这个方向在micro/nano-system和stem cell
proteomics结合的领域，现在交叉学科里面最活跃的方向之一。有两个职位，一个PHD
RA position, 一个postdoc fellowship，在University of Michigan的Mechanical
Engineering Dept，我都贴到下面了。如果有兴趣的请直接跟他联系。
http://me.engin.umich.edu/ibbl/employment.htm
New Graduate Research Assistant Opening in Nanofluidics and
Single-molecule Study
Description: The Integrated Biosystems and Biomechanics Lab (IBBL) at the
Mechanical Engineering Department of the University of Michigan, Ann Arbor
has an immediate openi... 阅读全帖 |
|
b*******k
发帖数: 16989 | 16 【 以下文字转载自 ChinaNews 讨论区 】
发信人: kingslandlee (sinodisiac), 信区: ChinaNews
标 题: Re: 金氏王朝在武汉大学的复制
发信站: BBS 未名空间站 (Tue Feb 21 05:15:06 2012, 美东)
黄从新语录
1.对下级：评选还没开始就知道结果
当2011年国家科技进步奖、973计划、国家十二五支撑计划还没有揭晓时黄从新在
全院大会上得意的说不出意外我今年就可以拿到国家科技进步奖二等奖一项、973计划
首席科学家一项、国家十二五支撑计划首席科学家一项。评选结果还没有公布，甚至评
选还没开始就宣布自己中了。
2.对无证行医的儿子：做手术死了人又不签你的名
黄鹤在2006年至2009年9月长达近4年的无证非法行医，并担任副主任医师期间，其
对临床是完全不懂的，其恐惧的问其父亲医死了人怎么办，黄从新指出做死了人又不签
你的名，你怕什么。结果每天的导管手术都安排没有经验的黄鹤上台期间医疗事故频发。
3.对媳妇：我是研究生院院长你怕什么
黄从新担任的另外一个职位是武汉大学研究生院院长，知情人士透露，其媳妇孙晶
认... 阅读全帖 |
|
b****n
发帖数: 8 | 17 Address:
Email:
Cell phone:
Work phone:
Oct 28, 2013
Department of Homeland Security
USCIS Nebraska Service Center
P.O. Box 87103
Lincoln, NE 68501-7103
I-140 Immigration Visa Petition Under First Preference EB-1
Petitioner/Beneficiary: XX Z., Ph.D.
Category of Petition: Alien of Extraordinary Ability
Classification Sought: 203(b)(1)(A)
Nature of Submission: Original Submission
Dear Immigration Officer,
This letter is respectfully submitted in s... 阅读全帖 |
|
b****n
发帖数: 8 | 18 Address:
Email:
Cell phone:
Work phone:
Oct 28, 2013
Department of Homeland Security
USCIS Nebraska Service Center
P.O. Box 87103
Lincoln, NE 68501-7103
I-140 Immigration Visa Petition Under First Preference EB-1
Petitioner/Beneficiary: XX Z., Ph.D.
Category of Petition: Alien of Extraordinary Ability
Classification Sought: 203(b)(1)(A)
Nature of Submission: Original Submission
Dear Immigration Officer,
This letter is respectfully submitted in s... 阅读全帖 |
|
S***A
发帖数: 40 | 19 DATE: Monday, April 7, 2014
TIME:
4:45 PM Registration
5:15 PM Professor Barry M. Trost, Tamaki Professor of Humanities and
Sciences, Stanford University, “The Alkyne Strategy For the Synthesis of
Bioactive Targets”
6:00 PM Networking and light dinner
7:00 PM Dr. Nicholas A. Meanwell, Executive Director, Medicinal
Chemistry, Bristol-Myers Squibb, “The Discovery of HCV NS5A Replication
Complex Inhibitors: Daclatasvir and Synergists”
PLACE: Knobbe ... 阅读全帖 |
|
x**x
发帖数: 92 | 20 我是在下面的网页看到的
http://www.bodybuilding.com/fun/ammonia.htm
quote:
our body uses amino acids for energy every day. There is no way to avoid
this. Your body constantly goes into catabolic (tissue breakdown) and
anabolic (tissue building) phases. When you accumulate mass (lean or fat),
your anabolic phases exceed your catabolic phases, but you still experience
both phases. When your body uses an amino acid for energy, it must convert
the amino acid to a useable form of energy.
It does this by strippin... 阅读全帖 |
|
l*********e
发帖数: 5483 | 21 很好，又长知识了
Evidence for attraction based on our immune system?
Sounds odd, but it seems to be true. Researchers studying the immune systems
of mice found that female mice would choose a mate whose MHC genes were the
least similar to her own. MHC stands for major histocompatibility complex.
These genes code for special protein markers that are attached to the
surface of cells and help the body recognize whether a cell belongs to an
organism or if it is an invader such as a bacteria or virus. If a c... 阅读全帖 |
|
a**********e
发帖数: 418 | 22 3. Please give me an example. I would like to know, and please demonstrate
how that example relate to our biological system
4. If you do not know me, please do not judge. Arrogance is not welcome
anywhere.
Based on my knowledge in organic chemistry, in nature there is no SINGLE
biologically active molecule could even possibly be made from inorganic
molecules without the presence of other organic molecules. Remember, nature
can not reproduce the laboratry conditions.
I will respond only toward a ... 阅读全帖 |
|
D*****r
发帖数: 6791 | 23 http://www.youtube.com/watch?v=hOLAGYmUQV0
[David Attenborough]
All life is related
And it enables us to construct with confidence
The complex tree that represents the history of life
Our planet, the Earth, is as far as we know
Unique in the universe; it contains life
Here plants and animals proliferate in such numbers
That we still have not even named all the different species
Darwin's great insight revolutionized the way in which we see the world
We now understand why there are so many differe... 阅读全帖 |
|
S*********g
发帖数: 24893 | 24 I just peed.
I peed out 1341134695243756294357625 water molecules.
Can you tell me what's the probability of peeing out 1341134695243756294357625 water molecules?
exactly 1341134695243756294357625 water molecules
not 1 more
not 1 less
what a miracle!
praise god
从古猿到人，在几百亿碱基对的基因上，这中间涉及了多少变化？要用最多１０万代的
过程完成，每一个变化都要恰到好处地发生，恰到好处地被保留。进化论，在宣称你自
己是客观实证的真理以前，请告诉我，这是什么样的概率？这个概率跟一个赌鬼连掷１０
万把六六顺的概率比起来如何？ |
|
g***2
发帖数: 403 | 25 Abbott Scientists Create One Molecule With Two Antibody Functions
First Combination of Large Molecules to Show Drug-like Properties and
Manufacturing Feasibility
Abbott Park, Illinois, October 18, 2007 — Abbott reported today that its
scientists are the first to discover a proprietary technology that combines
the function and specificity of two or more monoclonal antibodies (mAbs)
into one molecular entity that demonstrates drug-like properties and
manufacturing feasibility. These molecules, cal |
|
g****y
发帖数: 26 | 26 We are seeking highly motivated individuals for postdoctoral positions in
the Structural Biology Program of the Sloan-Kettering Institute, Memorial
Sloan-Kettering Cancer Center, NYC, USA.
The lab develops cutting-edge techniques (Nature,doi:10.1038/ nature09163 (
2010)) aimed at gaining a mechanistic understanding of fundamental
biological processes. Major emphasis is placed on development of novel
single-molecule detection and manipulation instrumentation that pushes the
envelop in sensitivity... 阅读全帖 |
|
w****g
发帖数: 44 | 27 If there are a lot of different types of A,B .... molecules in the system,
and I want to quickly get all the molecules of type A,do anyone know how can
I realize that ??
This is a kind of pattern matching problem ?
My thought is:
1. First transfer all molecule structures into a string and store them.
2. Then search for the strings for the desired string.
Any body has a better idea? or where can I find such an algorithm ?
Any clues are highly appreciated. |
|
l*****g
发帖数: 263 | 28 I suggest people researching in cell signaling check this site
regularly: http://www.signaling-gateway.org/
This is a joint project betwee AfCS (Alliance of Cellular Signaling) and
Nature. When you enter this site, you'll have a complete(!the most!)
description of over 3,000 signaling molecules. Well, you need to register
free to view the site.
For example, check specific molecules:
go to: http://www.signaling-gateway.org/molecule/
Search it with "calmodulin", you will find a list. Click calmodu |
|
d********r
发帖数: 303 | 29 First I would start by posting a figure on this board.
what is the blue molecule?
and what is the yellow one?
Answer:
Membrane protein is very difficult to crystallize. Therefore, Mackinnon spent
lots of time to get monoclonical antibody against KcsA potassium channel. The
blue molecule is the the Fab fragment and the yellow molecule is the channel.
The amazing thing is only the fab fragment interact each other and mediate the
crystallization. Therefore, the hydrophobic residues on the channel d |
|
D*********t
发帖数: 370 | 30 Well said (lmh and snoopy). Indeed there are no cancer-specific migration
inhibitors available as yet. However, cancer cells tend to overexpress
certain molecules and rely more heavily on these molecules for function (
including migration). The cancer-specific molecules (especially those
expressed on the cell surface) themselves are a focus of investigations, for
the purpose specific targeting. |
|
o2
发帖数: 2764 | 31 Ft, do you know the principle of x-ray diffraction? It requires the molecule
to be arranged in an orderly repeating pattern. So how you gonna arrange
this pattern by single molecule? The protein solution will have molecules
randomly arranged to all orientations, x-ray technique is not able to do
anything with it, it needs crystals.
virus
damage |
|
l**********n
发帖数: 240 | 32 Josef Martin Penninger
Most significant scientific contributions:
Our basic approach is to genetically manipulate and change genes in mice and
to
determine the effects of these mutations in development of the whole
organism and in
diseases. From these mutations we are trying to establish basic principles
of
development and basic mechanisms of disease pathogenesis. My laboratory
focuses on
heart diseases, autoimmune diseases and cancers, and bone diseases. On all
the listed
contributions below I ... 阅读全帖 |
|
g***j
发帖数: 40861 | 33 Tough Lessons From Golden Rice
Martin Enserink
It was supposed to prevent blindness and death from vitamin A deficiency
in millions of children. But almost a decade after its invention, golden
rice is still stuck in the lab
It's easy to recognize Ingo Potrykus at the train station in Basel,
Switzerland. Quietly waiting while hurried travelers zip by, he is holding,
as he promised, the framed and slightly yellowed cover of the 31 July 2000
issue of Time magazine. It features Potrykus's bearde... 阅读全帖 |
|
X******n
发帖数: 914 | 34 Protein precipitation is basically the removal of proteins from solution.
This can be achieved in many ways including the addition salt, which takes
away the water molecules surrounding proteins in solution (as the salt
concentration increases, more water molecules associate with the salt ions
instead of the proteins) and as a result the protein molecules come together
since less water is present between them. From this simple definition you
can generalize that protein precipitation does not imp... 阅读全帖 |
|
g****y
发帖数: 26 | 35 【 以下文字转载自 Postdoc 讨论区 】
发信人: galupy (Galoppogos-horse), 信区: Postdoc
标 题: postdoc opening@Sloan-Kettering Institute
关键字: postdoc opening,sloan kettering
发信站: BBS 未名空间站 (Sun Aug 14 14:28:02 2011, 美东)
We are seeking highly motivated individuals for postdoctoral positions in
the Structural Biology Program of the Sloan-Kettering Institute, Memorial
Sloan-Kettering Cancer Center, NYC, USA.
The lab develops cutting-edge techniques (Nature,doi:10.1038/ nature09163 (
2010)) aimed at gaining a mechanisti... 阅读全帖 |
|
p*l
发帖数: 1359 | 36 FRET is the best way to confirm interaction, although FRET between more than 2 molecules can be very tricky.
Super resolution imaging techniques can give you at best 20~30 nm resolution
in biological samples. With super resolution imaging, the best you can get
is to narrow down two molecules within 20~30 nm range. That's assuming you have a perfect system, fine tuned and operated by an optical scientist. At 20~30nm resolution, saying two molecules are interacting is a very good guess, but not 1... 阅读全帖 |
|
l**********1
发帖数: 5204 | 37 楼主 问题 解决了没?
若还没
DOCK 可以试一下这个 免费的 from UCSF:
//dock.compbio.ucsf.edu/
DOCK
DOCK addresses the problem of "docking" molecules to each other. It explores ways in which two
molecules, such as a drug and an enzyme or protein receptor, might fit together. Compounds which dock to
each other well, like pieces of a three-dimensional jigsaw puzzle, have the potential to bind. So, why is it
important to able to identify small molecules which may bind to a target macromolecule? A compound
which binds to a b... 阅读全帖 |
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t*m
发帖数: 4414 | 38 I won't agree with RY on drug development
Merck spends ~$8 billion on R&D each year (marketing and sales spending is a
lot larger than R&D), and it can get 2 new molecule drug approved each year
if lucky.
In China, 一类新药 doesn't mean new molecule drug. China never developed any new molecule drug. |
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d*****r
发帖数: 2583 | 39 【 以下文字转载自 Postdoc 讨论区 】
发信人: demoner (New York State of Mind), 信区: Postdoc
标 题: new postdoc/PhD RA position available at Univ of Michigan.
发信站: BBS 未名空间站 (Tue Jun 26 15:50:19 2012, 美东)
帮一个好朋友贴一个招人广告，这个方向在micro/nano-system和stem cell
proteomics结合的领域，现在交叉学科里面最活跃的方向之一。有两个职位，一个PHD
RA position, 一个postdoc fellowship，在University of Michigan的Mechanical
Engineering Dept，我都贴到下面了。如果有兴趣的请直接跟他联系。
http://me.engin.umich.edu/ibbl/employment.htm
New Graduate Research Assistant Opening in Nanofluidics and
Single-... 阅读全帖 |
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a*****x
发帖数: 901 | 40 原文摘抄：
"In order to illustrate the possible meaning of this result we shall suppose
that a charged molecule which has some special affinity for sodium may rest
either on the inside or the outside of the membrane but is present in
negligible concentrations elsewhere"..."From Boltzmann's principle, the
proportion of Pi of the molecules on the inside of the membrane..."
你认为里面的“a charged molecule”是什么？你是否坚持认为是钠离子? 其他的不做
评论了。 |
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t*********o
发帖数: 78 | 41 Postdoctoral Position in Single Molecule Enzymology : Columbus, OH, United
States
One postdoctoral position is available immediately in an enzymology
laboratory to elucidate the kinetics and dynamics of polymerization
catalyzed by human and viral DNA/RNA polymerases using single molecule Prism
-based or Objective-based Total Internal Reflection Fluorescence (TIRF)
apparatus. Background in single molecule technologies, computer programming,
enzyme kinetics, biophysics, and protein purification is... 阅读全帖 |
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H****s
发帖数: 301 | 42 I forget the source of information. It may be scientifically misleading, so
use this information at your own risk.
For GFP molecules in mammalian cells, the common ground is about 1000 GFP
molecules. Somebody ever claimed that 400 molecules were good enough. |
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b******y
发帖数: 627 | 43 Again, if A binds B strong, you can try native PAGE with increasing amount A
from zero to whatever maximum, the resolution of native gel might be
sufficient for you to distinguish B/Ai from B/A(i-1). If indeed, you will
get n+1 band on the native PAGE when combining all lanes: B, BA1, ... BAi,..
.. BAn.
Single molecule quenching might work as well. Label A with GFP with near 100
% efficiency with little quenching already. Establish complex B(A-GFP)n on
cover slide using TIRF. Do single molecule ... 阅读全帖 |
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b*******0
发帖数: 125 | 44 There has been a lot of controversy in recent years over the issue of
whether exogenous microRNA molecules can be absorbed from food and even have
a physiological effect. A new study using mouse models settles the question
by demonstrating that the posited dietary uptake does not take place. This
questions the potentially promising concept of creating functional foods
based on microRNAs.
The scientific world was astonished when, in 2011, Chinese researchers
claimed to have found evidence suggest... 阅读全帖 |
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z*t
发帖数: 863 | 45 其实如果我们换个方法脑洞一下，纯属假设，不要在我的帖子后面瞎回啊，我没有想替
老韩翻案的意思。
现在大家（包括我在内）都认为NgAgo用老韩的材料方法做不出来。
但是假设真菌体系里确实work了，而且不是在大于37度的条件下做出来的，那说明
NgAgo 在真菌里不需要高温也能起作用，而且说明NgAgo有可能需要assistantance
molecule才能起作用。而这个molecule很有可能是真菌里才有的，考虑到老韩实验室条
件简陋而且石家庄雾霾大，有没有可能他做出来的几次（假设他真看到了）是碰到了不
是特别严重的真菌污染？这样转细胞时顺便把真菌给弄死了，而且神奇的
assistantance molecule也一并进入细胞帮助了NgAgo的编辑？
最后再说一遍纯属娱乐，大家看着乐呵就行,不要把对老韩的不满发到我身上，嘿嘿 |
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z*******x
发帖数: 103 | 46 我看一下 1991文章（pdf）全文，Chen 的确是通讯作者。但，这不排除Chen 当时实在
最后一名作者手下干的。 下面信息是我从LINSLEY, PETER S.简历中读到的。至于chen
独立不独立我回答不了。只有少数人知道。
我说按诺奖规则Chen 没戏是 给予 Linsley是他当时的老板。
----------
1983-1996
Senior Scientist, Bristol-Myers Squibb Pharmaceutical
Research Institute, Seattle, WA
1996-1997
Director, Bristol-Myers Squibb Pharmaceutical Researc
h Institute, Seattle, WA
I discovered the B7/CD28/CTLA-4 T cell costimulatory axis.
In the late1980s, it was unclear how T cell
surface molecules, other than the antigen recept... 阅读全帖 |
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c**r
发帖数: 51 | 47 如果您指的是Bader的Atoms in Molecules, 大致看过一次，没注意到对这个题目的反复讨论，可能是我看得不细。用transferability作为关键词在index里面没找到相关页码。只发现在P133，5.2 Need for a quantum definition of atom里画了LiF,LiO,LiH的电子密度图，和一些文字说明，似乎Bader很注意对一个理论的构成元素的观察，他通过电子密度发现大概Li原子在这三个分子中有一些类似的分布，作为发展所谓 quantum definition of atom的一个动机。您能提供一下其他讨论的内容或者页码吗，谢谢
不过从P. Ayers的Atoms in molecules, an axiomatic approach. I. Maximum
transferability,JCP, 113,10886上发现了一些更加明确的观点。Ayers以公理的方式提出
Axiom T (maximal transferability): An atom in a molecule
is defined so that the p |
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o****u
发帖数: 1299 | 48 【 以下文字转载自 Faculty 讨论区 】
发信人: owlliu (小草莓), 信区: Faculty
标 题: ZZ: PhD/postdoc positions at ETH Zurich, Switzerland
发信站: BBS 未名空间站 (Wed Oct 20 12:13:23 2010, 美东)
One freind of mine who just became a Assistant Professor at ETH (Swiss
Federal Institute of Technology) Zurich is looking for PhD
students/post-docs for his research group in the field of attosecond
science. He is very young (<30), very smart (the most intellegient
yound sciendtest I've ever met) and employees at ETH are very well
paid (~... 阅读全帖 |
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P*****l
发帖数: 438 | 49 How many people in this article are in trouble now ?
Tiny transistor breaks new limits
Thursday, 18 October, 2001, 14:03 GMT 15:03 UK
Bell Labs also built the first ever transistor in 1947
Scientists in the US may have paved the way towards working molecular
computers.
A group of researchers at Bell Labs have made tiny functioning transistors a
million times smaller than a grain of sand.
Making large quantities of the tiny components should be straightforward
because they self-assemble.
But rese... 阅读全帖 |
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s*****l
发帖数: 1844 | 50 medimmune seems to have openings on the web...
Anyway, R&D in Pharma/Biotech is very tough nowadays. Now big pharma has
troubling developing small molecules, and layoff a lot of people working on
small molecules. Later, if they are not doing well on large molecules, I do
not know what they need to do next. |
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