z*********o
发帖数: 1091 | 1 插一个1.5 kb 的片断到pUC 载体中,克隆PCR 可以得到几个positive克隆, 而且在含有
抗生素的LB中生长正常,可就是都提不到质粒. 提取质粒的过程没有问题,因为同时提了
其他的质粒, 一切正常.有人遇到过这种情况吗? 真是太郁闷了! | a***e
发帖数: 1010 | 2 get everything clean, and do it again. it happens often. | z*********o
发帖数: 1091 | 3 Yes, actually I redid it from ligation step. I really could not understand
why all six clones did not work, but in the meanwhile, the other extracts
from different clones (different construct) worked well. I think the stuff
that I used should be clean. | I***a
发帖数: 13467 | |
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