s****6
发帖数: 34 | 1 Hello, Friends,
When I suspect one specific mRNA is a target of one microRNA, and I didn't
get a clue from those popular web-based target prediction tools. Can I
manually compare this microRNA with the 5'UTR and 3 UTR of that mRNA for
seed matching? What software could be used for this purpose?
Thanks a lot for any pointers! | X***n
发帖数: 366 | 2 Seed sequence is only about 6-nt, it's possible for it presents in mRNA
sequence by chance. Besides, seed sequence matching is not sufficient to
make a mRNA the target. You may try RNA22. You can loose the stringent by
allowing UN wooble and lower folding energy parameters. | s****6
发帖数: 34 | 3 This is very helpful to me. I appreciate it. |
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