a******n
发帖数: 392 | 1 如果要在细胞系里同时knockdown两个基因，怎么选择RNAi的方法阿？siRNA,
lentiviral shRNA? 效率哪种高？ | a***e
发帖数: 1010 | 2 Both siRNA and lentiviral shRNA methods are good enough to reach your
goal.
However, lentivirus usually has a higher infection efficacy than
transfection method. Therefore, using lentiviral shRNA may be a more
efficient
way to knockdown gene expression. | a******n
发帖数: 392 | 3 多谢。如果用lentiviral shRNA，infection 之后几天可以用puromycin测定
transduction的效率？ 在加puromycin之
前，需要把细胞重新plate吗？
【在 a***e 的大作中提到】
: Both siRNA and lentiviral shRNA methods are good enough to reach your
: goal.
: However, lentivirus usually has a higher infection efficacy than
: transfection method. Therefore, using lentiviral shRNA may be a more
: efficient
: way to knockdown gene expression.
|
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