w******e
发帖数: 1187 | 1 Tan group striked again...
http://www.pnas.org/content/early/2011/02/15/1016197108.abstrac
1. construct a conformation switch-based aptamer sensor which elicits
signal upon binding to specific target on cell(have been done many times);
2. use the sensor to quantify cancer cells down to a few hundred cells,
in both saline & serum, with a huge dynamic range (new to me, but
shouldn't be too hard)
3. use the sensor for in vivo imaging of cancer mouse model induced by
SC injected cancer cells. imaging is specific against one type of
cancer cells and specific for aptamer sequence.
(very similar to the first project I killed... chickened
out after a few tries-_-)
one major concern is they let the tumor grow to
1-2cm which seems way too big. combining w/ the fact that they killed
the mouse b4 imaging (is this the common practice? I know little about
imaging), kinda suspect the aptamer wouldn't work in live mice (clearance/
degradation won't allow the aptamer to reach tumor at such high level),
and aptamer wouldn't penetrate a more "normal" tumor w/ smaller size
and few lesions.
still, seems very nice work to me. anyone else care to comment?
P.S., is it common to do fluorescence imaging in mice? how "translatable"
is it to human, considering all the background and signal penetration issue? |
a********n
发帖数: 844 | 2 design上不是首例，functional assay信不信得过真是不好说，不过larry gold挺严格
吧 |
T**********t
发帖数: 1604 | 3 有没有人知道这些aptamer people的family tree啊。我就知道Aptamer大概最早是
Tuerk/Gold，Ellington/Szostak各自两个实验室开始搞的。后面怎么开枝散叶的就不
清楚了。Tan Weihong是师从什么人？最近几年的aptamer paper好像中国人很多啊，是
因为入门的门槛低么？ |
a********n
发帖数: 844 | 4 我是最末端的分支了，不过我老板是看paper自己起家的，要说门槛，自己起家的人绝
对是要细心耐心聪明加运气好。 |
a********n
发帖数: 844 | 5 我是最末端的分支了，不过我老板是看paper自己起家的，要说门槛，自己起家的人绝
对是要细心耐心聪明加运气好。 |
T**********t
发帖数: 1604 | 6 BSO跟了个细心耐心聪明加运气好的老板。:D
【在 a********n 的大作中提到】
: 我是最末端的分支了，不过我老板是看paper自己起家的，要说门槛，自己起家的人绝
: 对是要细心耐心聪明加运气好。
|
w******e
发帖数: 1187 | 7 sullenger是gold的postdoc，famulok是szostak的postdoc。john lis不知。
shi hua和版上某牛人是john lis的postdoc/phd。tan weihong是半路出家的化学牛
人。li yingfu不知。谁给补充一下？
尤其tan组，现在基本dominate cell-SELEX并搞了n种cell-based aptamer
application
，很不容易，很佩服。打江山的shangguan回国了，现在还没有大paper面世，可能在
攒ing。
aptamer paper虽多，大部分都是拿个thrombin aptamer搞各种sensor platform，不
厚道说其实是打酱油的哈哈~作应用的只有tan组、langer组、ellington组、lu组、
li组我比较看好。
【在 T**********t 的大作中提到】
: 有没有人知道这些aptamer people的family tree啊。我就知道Aptamer大概最早是
: Tuerk/Gold，Ellington/Szostak各自两个实验室开始搞的。后面怎么开枝散叶的就不
: 清楚了。Tan Weihong是师从什么人？最近几年的aptamer paper好像中国人很多啊，是
: 因为入门的门槛低么？
|
h*****o
发帖数: 342 | 8 他们并没有杀老鼠再做imaging，他们只是把老鼠麻醉了。
这个东西临床上不一定好用，你得测清楚什么时候是背景荧光，多长时间这些背景荧光
被排出去，多长时间真正的信号被降解掉。从他们的图上看，在老鼠上面好像注射两小
时后时间最好，在人身上是多少不好说。而且得做些免疫组化的片子来看看有imaging
信号的地方是不是真是癌组织。
不过他们用FACS能检出100多个癌细胞的那个应用挺酷的，也许对白血病的早期检测有
帮助吧。
【在 w******e 的大作中提到】
: Tan group striked again...
: http://www.pnas.org/content/early/2011/02/15/1016197108.abstrac
: 1. construct a conformation switch-based aptamer sensor which elicits
: signal upon binding to specific target on cell(have been done many times);
: 2. use the sensor to quantify cancer cells down to a few hundred cells,
: in both saline & serum, with a huge dynamic range (new to me, but
: shouldn't be too hard)
: 3. use the sensor for in vivo imaging of cancer mouse model induced by
: SC injected cancer cells. imaging is specific against one type of
: cancer cells and specific for aptamer sequence.
|
w******e
发帖数: 1187 | 9 你对，我英语单词没学好呵呵。好吧，我对tan组的敬仰又进了一步。。。
btw，1-2cm的tumor size你觉得正常吗？
imaging
这个体外应用感觉倒不难做。
【在 h*****o 的大作中提到】
: 他们并没有杀老鼠再做imaging，他们只是把老鼠麻醉了。
: 这个东西临床上不一定好用，你得测清楚什么时候是背景荧光，多长时间这些背景荧光
: 被排出去，多长时间真正的信号被降解掉。从他们的图上看，在老鼠上面好像注射两小
: 时后时间最好，在人身上是多少不好说。而且得做些免疫组化的片子来看看有imaging
: 信号的地方是不是真是癌组织。
: 不过他们用FACS能检出100多个癌细胞的那个应用挺酷的，也许对白血病的早期检测有
: 帮助吧。
|
h*****o
发帖数: 342 | 10 好像蛮巨大的，对小鼠来说
不过他们的imaging本来也没有要找出tumor的边界在哪
就是告诉你说他们的probe对肿瘤是特异的
【在 w******e 的大作中提到】
: 你对，我英语单词没学好呵呵。好吧，我对tan组的敬仰又进了一步。。。
: btw，1-2cm的tumor size你觉得正常吗？
:
: imaging
: 这个体外应用感觉倒不难做。
|
|
|
w******e
发帖数: 1187 | 11 my point is, w/ a tumor that big, wouldn't there be a lot of necrosis/
lesions going on, which greatly facilitate the tissue penetration, which
is THE problem when it comes to in vivo application?
【在 h*****o 的大作中提到】
: 好像蛮巨大的，对小鼠来说
: 不过他们的imaging本来也没有要找出tumor的边界在哪
: 就是告诉你说他们的probe对肿瘤是特异的
|
y****6
发帖数: 196 | 12 Optical imaging with NIR fluorephore is good for mouse imaging, especially
xenograft tumors. For human application, most likely it will be used in
optical guided surgery. |
y****6
发帖数: 196 | 13 It seems that Roger Tsien is working on optical guided surgery now. |
h*****o
发帖数: 342 | 14 他那个ACPP放大信号，所以比antibody或者aptamer做的标记好。我不是很了解后两者
用来做optical imaging的时候对比啊分辨率啊情况到底怎么样。antibody用来做PET的
比较多
【在 y****6 的大作中提到】
: It seems that Roger Tsien is working on optical guided surgery now.
|
h*****o
发帖数: 342 | 15 什么是thrombin aptamer，有没有入门文章看一下？
【在 w******e 的大作中提到】
: sullenger是gold的postdoc，famulok是szostak的postdoc。john lis不知。
: shi hua和版上某牛人是john lis的postdoc/phd。tan weihong是半路出家的化学牛
: 人。li yingfu不知。谁给补充一下？
: 尤其tan组，现在基本dominate cell-SELEX并搞了n种cell-based aptamer
: application
: ，很不容易，很佩服。打江山的shangguan回国了，现在还没有大paper面世，可能在
: 攒ing。
: aptamer paper虽多，大部分都是拿个thrombin aptamer搞各种sensor platform，不
: 厚道说其实是打酱油的哈哈~作应用的只有tan组、langer组、ellington组、lu组、
: li组我比较看好。
|
w******e
发帖数: 1187 | 16 aptamer against thrombin protein.
I guess you are interested in application of aptamer. Lu Yi, Andrew
Ellington,
Tan weihong, Li Yingfu each published an aptamer-sensor review last year.
you can start from there.
【在 h*****o 的大作中提到】
: 什么是thrombin aptamer，有没有入门文章看一下？
|
y****6
发帖数: 196 | 17 In many applications (mouse), NIR optical imaging can substitute PET. Eva M.
Sevick's group labeled Transtuzumab with both NIR dye and PET (or SPECT?)
agent for mouse tumor imaging. They conclude that NIR optical imaging
overperformed PET (or SPECT?) in many aspects. Cannot remember the details.
You can find their recent publciations if interested. |
h*****o
发帖数: 342 | 18 thanks!
【在 w******e 的大作中提到】
: aptamer against thrombin protein.
: I guess you are interested in application of aptamer. Lu Yi, Andrew
: Ellington,
: Tan weihong, Li Yingfu each published an aptamer-sensor review last year.
: you can start from there.
|
w******e
发帖数: 1187 | 19 good to know. thx for sharing!
M.
.
【在 y****6 的大作中提到】
: In many applications (mouse), NIR optical imaging can substitute PET. Eva M.
: Sevick's group labeled Transtuzumab with both NIR dye and PET (or SPECT?)
: agent for mouse tumor imaging. They conclude that NIR optical imaging
: overperformed PET (or SPECT?) in many aspects. Cannot remember the details.
: You can find their recent publciations if interested.
|
