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Biology版 - cysteine-rich protein oxidises and aggregates, how to prevent it?
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相关话题的讨论汇总
话题: dtt话题: oxidises话题: cysteine话题: prevent话题: aggregates
进入Biology版参与讨论
1 (共1页)
k******0
发帖数: 1073
1
I wanted to purify a protein, which is only stable for a couple day in DTT
and then started aggregation when DTT died out. How to prevent this, except
the change of DTT for TCEP?
B***Q
发帖数: 182
2
keep it anaerobically in the presence of DTT or TCEP?
k******0
发帖数: 1073
3
impractical

【在 B***Q 的大作中提到】
: keep it anaerobically in the presence of DTT or TCEP?
b******y
发帖数: 627
4
Upon purification, freeze in glycerol using liquid N2, store at -80, thaw it
when in use.
1 (共1页)
进入Biology版参与讨论
相关主题
Re: 1st paper --random journal club蛋白多聚
Re: biological word translationin western for a 25kd protein, the band is at 50kd
paper help JBC请教2个western的试剂的问题
关于 membrane receptor 是monomer,dimer,还是multimerTEV切蛋白
求救,DTT和TCEP在蛋白质稳定性的作用Trypsin EDTA
问一个二硫键形成的实验问题EMSA遇aggregate--衰人再问
请教buffer里的各种成分作用再问纯化蛋白的问题。size exclusive column 完全没有retension.
请教DTT能打开链间二硫键吗?急! 请教做protein crystallography的大牛,包子答谢!
相关话题的讨论汇总
话题: dtt话题: oxidises话题: cysteine话题: prevent话题: aggregates