d********r
发帖数: 3279 | 1 需要写一个关于 using optogenetics in invertebrates 的 Perspective review
大家有什么点子没？
what's going on in this field now?
what are people doing?
where is this all going?
谢啦！ | s********x
发帖数: 472 | 2 基本都是再copy vertibrate 的技术。虽然最早尝试过改造rhodopsin。
做的最多的应该是c elegans，透明好做。而且每个neuron都研究过了。
这方面的reveiw太多，看看最新review，再吧最近的paper summarize 就不错，要有独
到的见解太难了。 | d********r
发帖数: 3279 | 3 嗯，谢谢你的comment，不过说基本都是 copy vertebrate 这个不对吧？animal level
最先是在 C.elegans 试的，后来才其他动物。
【在 s********x 的大作中提到】
: 基本都是再copy vertibrate 的技术。虽然最早尝试过改造rhodopsin。
: 做的最多的应该是c elegans，透明好做。而且每个neuron都研究过了。
: 这方面的reveiw太多，看看最新review，再吧最近的paper summarize 就不错，要有独
: 到的见解太难了。
| d********r
发帖数: 3279 | 4 My thoughts so far:
I think the "uniqueness" of using optogenetics in invertebrates, worms in
particular, is still essentially related to the general advantages of using
this model organism in the first place: transparent, small size, simple
nervous system and genetic screens.
For ephys analysis of synaptic function in dissected preps using
optogenetics, there is no big difference between worms and mice. For whole
animal behavioral studies under light stimulation, worms under a LED is a
lot easier than mice wearing optic fiber implants.
Coupling behavior with EM ultrastructure of course is a unique application
for small transparent invertebrates. You can still use brain slices and
retinal preps etc. to couple stimulation with EM, but there is no direct
link to behavior.
Forward genetic screens with optogenetic stimulations: it seems lots of
people are thinking about this but I am not aware if anyone has done so and
what's the current status.
Neuron-behavior mapping: This is a big one I think. Fly people has been
using optogenetics to randomly stimulate thousands of neuron lines for
activity mapping (Vogelstein et al. 2014 Science). Gaby Maimon lab at
Rockefeller is also doing similar stuff for particular behaviors such as
navigation. C. elegans using cell specific promoters is more straightforward
in this regards. Worm people is thinking about generating strains with
single neuron expression for all 119 neuron classes, so that behavior,
calcium dynamics and even ephys properties can eventually be studied at the
cellular level for the entire nervous system. Such task is certainly only
feasible for worms. |
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