h*****G
发帖数: 113 | 1 By ectopic expression of only 4 transcription factors, terminal
differentiated somatic cells can be transformed to be iPS cells. My question
is that if iPS cells or ES cells can be directly programmed to a specific
somatic cell fate by a pool of lineage specific transcription factors
without usually used stepwise protocols. If yes, could you please show me
some of the references? If not, what do you think might be the problem? | w*e
发帖数: 740 | 2 在再生医学里面，不一定非要用ES，IPS细胞
有时候用临近的，同一个LINEAGE的其他类型的细胞，直接通过几个因子就可以相互转换
好象胰腺里面搞的比较火热，DOUGLAS MELTON 08年的NATURE 就是类似的
还有 从ES细胞到终末分化的细胞，并不单单是转录因子的作用，
有时候细胞间的相互影响作用也很大
question
【在 h*****G 的大作中提到】
: By ectopic expression of only 4 transcription factors, terminal
: differentiated somatic cells can be transformed to be iPS cells. My question
: is that if iPS cells or ES cells can be directly programmed to a specific
: somatic cell fate by a pool of lineage specific transcription factors
: without usually used stepwise protocols. If yes, could you please show me
: some of the references? If not, what do you think might be the problem?
| h*****G
发帖数: 113 | 3 Thank you.
Lineage reprogramming is definitely very promising. As mentioned in another
post, it has been showed that fibroblast can be transformed to neuron
with only 3 TFs.
At the same time, direct programming from ES/iPS cells to somatic cells are
few. And I was not be able to find any publications. So was wondering if
someone can help me with some papers about direct programming from ES/iPS
cells to somatic cells with TFs, not stepwise extracellular signals.
Thanks.
转换
【在 w*e 的大作中提到】
: 在再生医学里面，不一定非要用ES，IPS细胞
: 有时候用临近的，同一个LINEAGE的其他类型的细胞，直接通过几个因子就可以相互转换
: 好象胰腺里面搞的比较火热，DOUGLAS MELTON 08年的NATURE 就是类似的
: 还有 从ES细胞到终末分化的细胞，并不单单是转录因子的作用，
: 有时候细胞间的相互影响作用也很大
:
: question
| t****p
发帖数: 1504 | 4 These are good questions! I also thought about that.
Theoretically, directional differentiation is easier than reprogramming,
because the latter is not a natuarl process. There is no any reason that
transcription factors should not force cells to differentiate into specific
lineage, given the facts of iPS.
I don't think any lab has achieve this, though.
question
【在 h*****G 的大作中提到】
: By ectopic expression of only 4 transcription factors, terminal
: differentiated somatic cells can be transformed to be iPS cells. My question
: is that if iPS cells or ES cells can be directly programmed to a specific
: somatic cell fate by a pool of lineage specific transcription factors
: without usually used stepwise protocols. If yes, could you please show me
: some of the references? If not, what do you think might be the problem?
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