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发帖数: 130 | 1 Methods Mol Biol. 2014;1119:239-65. doi: 10.1007/978-1-62703-788-4_14.
Techniques for characterizing cytomegalovirus-encoded miRNAs.
Hook LM1, Landais I, Hancock MH, Nelson JA.
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Abstract
microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at
the posttranscriptional level, by binding to sites within the 3'
untranslated regions of messenger RNA (mRNA) transcripts. The discovery of
this completely new mechanism of gene regulation necessitated the
development of a variety of techniques to further characterize miRNAs, their
expression, and function. In this chapter, we will discuss techniques
currently used in the miRNA field to express, detect, and inhibit miRNAs as
well as methods used to identify their targets.
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