p**i
发帖数: 3525 | 1 已经试了1x, 2x SDS sample loading buffer，都丝毫无效
谢谢了先? | s****a
发帖数: 436 | 2 试一下glycine呢？
【在 p**i 的大作中提到】
: 已经试了1x, 2x SDS sample loading buffer，都丝毫无效
: 谢谢了先?
| a***e
发帖数: 1010 | 3 how do you know your proteins are on the beads?
how about biotin competitive elution?
【在 p**i 的大作中提到】
: 已经试了1x, 2x SDS sample loading buffer，都丝毫无效
: 谢谢了先?
| s****a
发帖数: 436 | 4 Purification of biotinylated proteins on streptavidin resin: A protocol for
quantitative elution。
For elution of biotinylated proteins, 500 mL
release solution (2% SDS, 30 mM biotin, 50 mM phosphate,
100 mM NaCl, 6 M urea, 2 M thiourea, pH ,12)
were added to the resin and incubated for 15 min at RT,
followed by incubation for 15 min at 96C. Afterwards, the
resin was pelleted by centrifugation for 5 min at 16 100g
【在 p**i 的大作中提到】
: 已经试了1x, 2x SDS sample loading buffer，都丝毫无效
: 谢谢了先?
| n******d
发帖数: 1055 | | p**i
发帖数: 3525 | 6 1
【在 n******d 的大作中提到】
: 我有答案，绝对管用，但需要包子。你有多少？
| s******y
发帖数: 28562 | 7 This is the correct protocol to be used.
for
【在 s****a 的大作中提到】
: Purification of biotinylated proteins on streptavidin resin: A protocol for
: quantitative elution。
: For elution of biotinylated proteins, 500 mL
: release solution (2% SDS, 30 mM biotin, 50 mM phosphate,
: 100 mM NaCl, 6 M urea, 2 M thiourea, pH ,12)
: were added to the resin and incubated for 15 min at RT,
: followed by incubation for 15 min at 96C. Afterwards, the
: resin was pelleted by centrifugation for 5 min at 16 100g
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