m******p
发帖数: 67 | 1 i have a sample, either total RNA or cDNA. anyone has a method to determine
whether it is RNA or cDNA?
Thanks |
s******y
发帖数: 28562 | 2 If you know the sequence you can use a pair of primers and the taq PCR
polymerase. The taq polymerase does not use RNA as template in an usual PCR
condition.
determine
【在 m******p 的大作中提到】
: i have a sample, either total RNA or cDNA. anyone has a method to determine
: whether it is RNA or cDNA?
: Thanks
|
m**********n
发帖数: 299 | 3 Use NanoDrop.
A260/A280, A260/A230;
See the reference for details.
http://batzerlab.lsu.edu/genomics/documentation/3130_NanoDrop_t |
m**********n
发帖数: 299 | 4 The simplest method is the best method.
PCR
【在 s******y 的大作中提到】
: If you know the sequence you can use a pair of primers and the taq PCR
: polymerase. The taq polymerase does not use RNA as template in an usual PCR
: condition.
:
: determine
|
s******y
发帖数: 28562 | 5 看了以下连接里面的内容，好像是说２３０的吸收度和buffer 有关系？
如果楼主不知道buffer 是什么的话恐怕很难办吧？
【在 m**********n 的大作中提到】
: Use NanoDrop.
: A260/A280, A260/A230;
: See the reference for details.
: http://batzerlab.lsu.edu/genomics/documentation/3130_NanoDrop_t
|
m******p
发帖数: 67 | 6 smart methods. pcr and od. thanks. |
s*******e
发帖数: 1010 | 7 add DNAase/RNAase and run a gel?
What if you have a mixed sample? |
s******y
发帖数: 28562 | 8 这个也可以，如果样品比较多的话。
【在 s*******e 的大作中提到】
: add DNAase/RNAase and run a gel?
: What if you have a mixed sample?
|
m******p
发帖数: 67 | 9 wa.. another smart idea. |
