j*****a
发帖数: 92 | 1 阴离子交换色谱 Q Sepharose Fast Flow (GE Healthcare Life Sciences)pH
stability Working Range 2-12 .用1 M NaOH 洗,用纯水洗到pH 7, 换TrisHCl
buffer pH 5.5 平衡, elute pH 变成9.洗了一天还是pH 9. 有人遇到过吗？ | s********n
发帖数: 2939 | 2 You column is stable between 2-12. However, you washed it by 1 M NaOH?!
And why did you use Tris-HCl for pH 5.5? Tris-HCl also works between 7-9.
Did you measure the pH offline or online? If online, did you calibrate the
pH sensor? | j*****a
发帖数: 92 | 3 Good point. I need to switch buffer system. | N2
发帖数: 81 | 4 不要被误导, 是要用强碱强酸洗的,等会有空写给你protocol
【在 j*****a 的大作中提到】
: Good point. I need to switch buffer system.
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