h*********g
发帖数: 18 | 1 After gel extraction, I checked the DNA using Nanodrop, the 260/280 is 1.5,
260/230 is 0.5. Can I still use the DNA? I will use the DNA as template to
make RNA. Thanks. |
m*********D
发帖数: 1727 | 2 Elute by ddH2O? pH could affect the ratio. So use TE buffer to measure O.D.
again. |
h*********g
发帖数: 18 | 3 Yes, I eluted by ddH2O. So I should use TE buffer to elude? I will use it to
make RNA. Can I still use the DNA to make RNA? Thanks.
.
【在 m*********D 的大作中提到】
: Elute by ddH2O? pH could affect the ratio. So use TE buffer to measure O.D.
: again.
|
s********r
发帖数: 312 | 4 probably not suitable for subsequent experiments. Is the DNA concentration
normal? One possibility is ethanol carryover. |
x********e
发帖数: 35261 | 5 是有这个现象。做连接没问题，转录就不确定了。
我一般不用nanodrop测，直接跑胶看回收质量
,
【在 h*********g 的大作中提到】
: After gel extraction, I checked the DNA using Nanodrop, the 260/280 is 1.5,
: 260/230 is 0.5. Can I still use the DNA? I will use the DNA as template to
: make RNA. Thanks.
|
m*********D
发帖数: 1727 | 6 Yes,the DNA should be fine for transcription template. If you load on a
gel, you may even see ssDNA bands.
Better elude in TE. However if elude in ddH2O, you can add some NaCl
solution to 100 mM.this helps to form dsDNA. |
