n********n
发帖数: 8336 | 1 The "Gnostic" sects
https://en.wikipedia.org/wiki/Gnosis
Among the gnostics, gnosis was first and foremost a matter of self-knowledge
, which was considered the path leading to the goal of enlightenment as the
hidden knowledge of the various pre-Judeo-Christian pagan Mystery-Religions.
[14] Knowledge that first relieved the individual of their cultural
religious indoctrination and then reconciled them to their personal deity.[
14] Through such self-knowledge and personal purification (virtuous l... 阅读全帖 |
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t********e
发帖数: 48 | 2 You did not understand what I was talking about...
Yes...Hinaya was state religion in these countries and had more political
and social engagements..but whether this was positive or negative is very
questionable, at least it did not lead to the purification of these
societies.
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J******s
发帖数: 7538 | 3 I read almost 80 pages out 398 pages of the English version, and so far
I can understand it. As for me, English version is easier to understand,
and I do not know what the so called 十六观智 are. I am in the third part
of this book, and it is about ' the way into Vipassana Mediattion'.
If possible I will read the Chinese version later, the problem for me to
read Chinese version is there are such a lot concepts, which I can not
understand.
Thanks for ur suggestions!
I will read the English version of... 阅读全帖 |
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J******s
发帖数: 7538 | 4 我学佛从来没有想过一定要成佛。
甚至解脱对我都太遥远，但是我觉得学佛法，meditation自己的思想以及行为确实可以得到
purification，我觉得这个值得做。
从我目前学习的体会，我觉得佛法给我一个更加开阔的世界观，对认识自己，认识世界
都很有帮助。 |
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J******s
发帖数: 7538 | 5 每个人活着有自己的目标和理想的，你想涅磐不见得谁都想涅磐。
而且你不是很懂得轮回吗，初果还得七世轮回呢。
你为什么总是希望别人按着你的意志行事？
目前不以解脱为目的是我自己的业。
你想解脱是你的事儿。而且我目前确实不想那么远，我不是monk或者nun，修行不是我
毕生唯一要做的事情。佛讲究因缘。
并且我没看出来你真的想解脱，你所谓的解脱更像是口号。你也不是以弘扬佛法为目的，
而且你的态度和方式根本宣传不了什么佛法。你怎么不跟沈家桢居士学学。
佛法就是真灭亡了，我觉得你也证不了涅磐。
佛法很有意思，佛法展示的认识这个世界的方式我接受，而且佛法明确说了学佛就是
戒定慧的不断purification。 |
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J******s
发帖数: 7538 | 6 不是的mm，我打算去修行的，真正尽量放下有常和有我。我觉得这种purification非常
好，是值得做的。佛说transformation is irreversible. |
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J******s
发帖数: 7538 | 7 八正道
正语经文辑要
汤华俊 编辑翻译 2005年7月
英文原文网址：Right Speech
The definition
"And what is right speech? Abstaining from lying, from divisive speech, from
abusive speech, & from idle chatter: This is called right speech." — SN
XLV.8
定义：
诸比丘!云何为正语耶?诸比丘!离虚诳语、离离间语、离粗恶语、离杂秽语。诸比丘!此
名之为正语。
——《相应部*大篇*道相应*无明品*分别》
Five keys to right speech
"Monks, a statement endowed with five factors is well-spoken, not ill-spoken
. It is blameless & unfaulted by knowledgeable people. Which five?
"It is spoken at the right time. ... 阅读全帖 |
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J******s
发帖数: 7538 | 8 wuyu check this please，it is a nice book based on Visuddhimagga or The Path
of Purification by Buddhagosha and also explains other Sublime States.
http://www.buddhanet.net/pdf_file/allmetta.pdf
Loving-kindness meditation by Ven.Sujiva
:-) |
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J******s
发帖数: 7538 | 9 wuyu check this please，it is a nice book based on Visuddhimagga or The Path
of Purification by Buddhagosha and also explains other Sublime States.
http://www.buddhanet.net/pdf_file/allmetta.pdf
Loving-kindness meditation by Ven.Sujiva
:-) |
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Y**u
发帖数: 5466 | 10 ☆─────────────────────────────────────☆
wuyu (wuyu) 于 (Thu Sep 22 11:39:16 2011, 美东) 提到:
请各位继续批评指正，谢谢！
第三篇：慈心念
慈心念的在修行中最重要修行之一。我个人认为，慈心念，安般念，身心受念住，应该
是初学者修行的三个支柱。佛法修行中，除了以解脱为目的的念住修行是佛家独有外，
其他的业处在别的入世宗教中都可能会有类似的方法。所以，有人如果认为“ 慈心念
”类似基督教里祷告，我也赞成。慈心禅练习的内容我已在第二章安般念的前方便中讲
过，此处不在赘述。一般我会在安般息念前，睡前做全套练习。平时则只以柔软慈爱的
心态祝福境遇所缘“快乐智慧无烦恼”或“福慧圆满”。佛法里对慈心禅作用有明确定
位。根据心法及心所法我们还可以对慈心禅的法理进行推测。
对于念住修行的“观禅”，作为“止禅”的“慈心念”和“安般念”所培养的定力是必
不可少的。慈心禅的独特作用还表现在他是在“轮回”中最好的保护法之一。我们知道
，慈悲也是”四梵住”的组成部分，即入世修行的最高层”梵天”所持有的品质。佛法
认为它的功... 阅读全帖 |
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r**m
发帖数: 1825 | 13 网上看到这个， 如果fonts有问题请看原link
http://www.udaya.dhamma.org/C_JCBSSL4Vipassana.html
[首頁/法的開示/烏巴慶內觀禪修之古老根源]
烏巴慶內觀禪修之古老根源*
Bhikkhu Anālayo
烏巴慶（U Ba Khin）所傳的內觀禪修已成為當今世上最廣為修行的內觀法之一。這要
特別感謝葛印卡的努力不懈，使烏巴慶的方法無論是在美國般的富裕社會或在印度的貧
困地區，都一樣是免費的佈施教導；而無論是在斯里蘭卡等上座部佛教國家或在杜拜、
伊朗的回教國家[1]，方法指導也都一樣。這種內觀禪修法也可以在東方及西方的監獄
裡學到，因為烏巴慶的方法甚至能感化殘酷罪犯，而這已獲得政府的認可。
這樣的成就說明了此方法有可能開展解脫洞見，然而我們對於這個禪修方法的根源卻所
知不多。烏巴慶（1899 - 1971）從烏鐵（U Thet 1873 - 1945）學得內觀禪修，而烏
鐵則是在著名的緬甸學問僧雷迪大師[2]（Ledi Sayadaw 1846 - 1923）的支持下開始
教授。除此 之外，緬甸方面似乎並無更多資料。雖然緬甸的記錄如... 阅读全帖 |
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f*****y
发帖数: 2 | 14 Ph. D. Graduate Student Position is available immediately at
the University of Connecticut, NMR laboratory Storrs,
Connecticut, U. S. A.
A graduate student (or a transfer graduate student) will be
offered with a Research Assistantship towards Ph. D. in
Pharmaceutical Science. Student who has
a biochemistry background (especially experience in protein
expression and purification) is preferred. Candidate should
have good communication skills in
English. You will have an opportunity to learn modern |
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f*******c
发帖数: 94 | 15 For this topic, why not ask me, in fact I am specialist in this area (
bioseparation). For Heparin，small MW heparin is not easy to be obtained
with high purity, it need specific enzyme digestion and downstream affinity
purification, virus need be clarified by membrane filtration and solvent
treatment. In addition recombinant heparin and synthesis heparin did not
show similar bioactivity or therapeutic results in clinic I and II test.
Heparin is a sugar with multiple SO3H group, it is not peptide |
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A****A
发帖数: 270 | 16 【 以下文字转载自 Boston 讨论区 】
发信人: AGATHA (Agatha Christie), 信区: Boston
标 题: 【活动】复旦交大校友会联合主办：“中国好才艺”中秋联欢会！
发信站: BBS 未名空间站 (Mon Sep 24 23:56:59 2012, 美东)
亲爱的朋友们：
值此中秋佳节之际，复旦与交大校友会诚挚邀请您来参加我们共同主办的“中国好才艺
”中秋联欢会！如果您在业余喜欢呼朋唤友去卡拉OK，或者擅长乐器舞蹈或书画，又或
者会说评书爱讲相声……任何才艺都不要错过这次表现机会！
无论您是否毕业于复旦或者交大，我们都期待您的到来，届时我们将提供卡拉OK音响等
设备，并提供月饼茶点水果饮料，所有现场观众都自动获得现场评委资格，还将获得两
件免费T-shirt！身怀绝技的当然要一显身手赢大奖，平凡如我的正好能聊天游戏叙友
情。
本次“中国好才艺”中秋联欢会将借鉴“中国好声音”的评审办法：现场四位评委转身
之后，每位可为选手打分最高至20分，点评之后由所有现场观众投票，加总后得分高者
将当场获得由复旦校友赞助的精美奖品。请您在报名时填写清楚您的才艺内容，... 阅读全帖 |
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A****A
发帖数: 270 | 17 6月14-15日 复旦人相约查尔斯河----后援团火热招募中
波士顿龙舟赛是波士顿华人一年一度的体育盛事，今年的第35届龙舟赛将首现上海复旦
大学代表队！这支年轻的队伍将在本周末（6月14-15日）首航查尔斯河！两轮分组赛将
于周六在MIT河段进行，紧张刺激的决赛在周日的Harvard河段展开角逐。在NeoBiolab
和NovoProtein 两家生物公司及“佛伦汉服”的联合赞助下，复旦队员将身着传统汉服
集体出战！届时我们在沿河观战绝佳位置有展位及休息区，现诚招热情如火的你加入我
们的后援团为复旦加油助威。请联系 y**********[email protected] 加入我们复旦后援团。
比赛具体赛程及节目请参见如下官方网站：
http://www.bostondragonboatracing.org 龙舟节是波士顿地参与人数最多覆盖范围最广的华人年度文化盛事，将有众多团体沿河亮相，提供丰富多彩的文艺表演，食品品尝，游戏抽奖等形式多样丰富多彩的有趣活动，欢迎带上家人，叫上朋友，一起来度过一个难忘的愉快周末！别忘了，我们复旦校友会不仅派出了我们的史上首只参赛队伍，也有展位和休息区供和新老校友... 阅读全帖 |
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s*****e
发帖数: 970 | 18 This is Jon Tilly's comment......
Molecular Human Reproduction
Volume 15, Issue 7, 2009, Pages 393-398
Purification of germline stem cells from adult mammalian ovaries: A step
closer towards control of the female biological clock?
Tilly, J.L.a b , Telfer, E.E.c
a Vincent Center for Reproductive Biology, Vincent Obstetrics and
Gynecology Service, Massachusetts General Hospital, Boston, MA, United
States
b Department of Obstetrics, Gynecology and Reproductive Biology, Harvard
Medical School, Bo |
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a****y
发帖数: 1035 | 19 【 以下文字转载自 Military 讨论区 】
发信人: sunnyday (艳阳天), 信区: Military
标 题: 我国已成功实现太阳能冶炼高纯硅
发信站: BBS 未名空间站 (Wed Jan 20 12:03:46 2010, 美东)
据2009年《中国物理快报》刊登的《Silicon Purification by a New Type of Solar
Furnace》一文报导：由中国科技大学、中科院物理所发展的新技术，已宣告成功。
太阳能级高纯硅，SoG Silicon（注：硅纯度至少要达到6个9，或杂质含量不得高
于百万分之一，PPM量级），是当前光伏产业最广泛应用的原材料。通常由西门子法生
产，但耗能过多，污染严重。有不少学者建议用直接纯化的冶金法取代西门子法，实际
上仍严重耗能，仅达5个9的纯度。有文献倡议用太阳能冶炼高纯硅，但或者由于太阳炉
成本太高、温度太低，或由于所选冶炼过程污染严重，均未获成功。
近来，陈应天教授等人应用他们所发明的无光象主动光学理论，廉价而成功地制作
了一种新型的太阳炉。能有效地稳定地将10000倍或更高一些的太阳光聚焦在小网球大 |
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M**a
发帖数: 4816 | 20 Postdoctoral and medical fellow positions are available to participate in
ongoing studies of molecular and cellular neurobiology in developmental and
aged related brain disorders as well as clinical biomarker searching/
validation in Alzheimer’s disease. Candidates with a strong background in
molecular biology, including molecular cloning, vector design and
construction, enzyme assays and purification or cell biology, including
primary cell cultures of neurons, glia, endothelial cells and stem c |
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Z****Q
发帖数: 13 | 21 I graduated from zheda in 2002 with a master degree. Now i am at the end to
finish my ph.d study. My major is biochemical engineering, research field is
microbial fermentation and protein purification. If you know any available
opening in this area, could you please tell me or help me to distribute my
resume. Thanks a lot. I really appreciate your help. My contact information
is: cell (330)-990-1373. my e-mail: z***********[email protected]. |
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M**a
发帖数: 4816 | 22 Postdoctoral and medical fellow positions are available to participate in
ongoing studies of molecular and cellular neurobiology in developmental and
aged related brain disorders as well as clinical biomarker searching/
validation in Alzheimer’s disease. Candidates with a strong background in
molecular biology, including molecular cloning, vector design and
construction, enzyme assays and purification or cell biology, including
primary cell cultures of neurons, glia, endothelial cells and stem c |
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g***m
发帖数: 465 | 23 man, refer to gzip's post.
the ligation is somehow vulnerable.
1. check with your lab fellow to make sure your ligase is still alive.
2. always use latest ligation buffer, you can even smell the thio stuff if you
are using NEB enzyme.
3. critical thing is your insert: never directly use the insert purified from
gel, you need gel purification followed by ethanol precipitation to make sure
the purity. However, you can use gel extracted vector for ligation in small
volume.
4. add enough insert, for |
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M****e
发帖数: 70 | 24 is there IP assay available? i think it is necessary if you
can fractionate the protein by columns (many biochemcal stuffs
that i am not familiar, which i read from purification of
p300 transcription factor, i think people in this area knows
a lot). then do the assay to see which fraction has the
activity (is this specific reaction?) thus you know the effective
size.
is this endogenous or expressed protein? guess it is
endogenous, given the big size. try IP with the antibody and
do the assay. or |
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r****o
发帖数: 105 | 25 1 design primers of ~30 bp length.
2.Use good polymerase to do PCR (herculase from stratagene is great,
a Pfu with special buffer in it to increase the fidelity)
3. add 1~3 ul Dpn I directly to the PCR reaction tube. digest it
for at least 3 hours in 37 degree
4.Desalt the PCR sample with PCR purification kit from Qiagen.
5.Transform competent E.Coli.
6. Pick up several colonies , grow small cultures and do miniprep.
7. Send the DNA to sequencing. |
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r****o
发帖数: 105 | 26 1 design primers of ~30 bp length.
2.Use good polymerase to do PCR (herculase from stratagene is great,
a Pfu with special buffer in it to increase the fidelity)
3. add 1~3 ul Dpn I directly to the PCR reaction tube. digest it
for at least 3 hours in 37 degree
4.Desalt the PCR sample with PCR purification kit from Qiagen.
5.Transform competent E.Coli.
6. Pick up several colonies , grow small cultures and do miniprep.
7. Send the DNA to sequencing. |
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M****e
发帖数: 70 | 27
i do not think that everybody has read this paper. we need more enthuisasm and
effort to keep active discussion and make this MCBJC run. meanwhile, think
about the paper that you will pick up later.
cause
these unknown proteins could interact with separase/securin and thus make the
result hard to interpret. an affinity purification might help, however, it
still
could not exclude contamination of tightly associated proteins. yet the major
goal for this part of work is to identify "inhibitory fac |
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r****o
发帖数: 105 | 28 几个小建议：
(1) 去磷酸化不是那么重要。
(2)换新的连接酶尤其是新的BUFFER。连接反应的BUFFER
非常容易坏，因为里面有ATP，冻融几次就不行了。
(3)载体和插入片段的量要足够。建议插入片段的量越多
越好。我一般让片段的莫尔量是载体的几十倍。
(4)既然你用琼脂糖凝胶纯化你的片段。我估计你用UV照射过
胶来确定位置，然后切下有DNA的部分，是吧？UV照射下，
很容易让DNA形成嘧啶二聚体，急剧降低连接效率。所以你要么
不用gel purification，如果必须分离两个片段，你可以
同时在两条LANE上跑少量的DNA和大量的DNA，切下少量DNA的
胶用UV照射确定所要片段的位置。 |
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M****e
发帖数: 70 | 30 my point is to make sure that you have transfer of good
quality and quantity of RNA onto the blotting material.
the washing stringency for your experiment is not quite
high (i.e., 42C). i think the problem is mainly due to
bad RNA or probe. if there is a lot of radioactive
nucleotides incorporated into the probe, you should
detect very hot signal after purification. denature the
probe before use. GAPDH is a housekeeping gene that is
typically used for normalization of load. i suggest you
read a |
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m**o
发帖数: 13 | 31 I am not so sure if RNase keep active in such a solution containing 50% or so
isopropanol. However, For purification purpose, it is not suggested to have a
nuclear acid precipitated with isopropanol method either under low temperature
or for long incubation. Salts often have lower solubility in isopropanol
solution than in ethanol solution. |
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l**********1
发帖数: 5204 | 32 The corresponding author of below Barcelona one PhD thesis (2009)
its reference list CNS only limited:
link:
//www.tesisenred.net/bitstream/handle/10803/7182/tjsc.pdf?sequence=1
i.e.
M.Reza Ghadiri, Ph.D
//www.ncbi.nlm.nih.gov/pubmed/19520909
and
//www.ncbi.nlm.nih.gov/pubmed/19566101
J Am Chem Soc. 2009 Jul 8;131(26):9368-
Universal translators for nucleic acid diagnosis.
Picuri JM, Frezza BM, Ghadiri MR.
Source
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines R... 阅读全帖 |
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l**********1
发帖数: 5204 | 33 The corresponding author of below Barcelona one PhD thesis (2009)
its reference list CNS only limited:
link:
//www.tesisenred.net/bitstream/handle/10803/7182/tjsc.pdf?sequence=1
i.e.
M.Reza Ghadiri, Ph.D
//www.ncbi.nlm.nih.gov/pubmed/19520909
and
//www.ncbi.nlm.nih.gov/pubmed/19566101
J Am Chem Soc. 2009 Jul 8;131(26):9368-
Universal translators for nucleic acid diagnosis.
Picuri JM, Frezza BM, Ghadiri MR.
Source
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines R... 阅读全帖 |
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f******s
发帖数: 115 | 34 可是qiagen说明书有这么一句话：
6. After storage, purify RNA using a QIAGEN kit (see Table 1, page 8).
Be sure to remove tissues from RNAlater RNA Stabilization Reagent prior to
disruption and homogenization in the RNA purification procedure. If tissues
were stored at –20°C, remove any crystals that may have formed.
他们说的”remove any crystals that may have formed“到底是往哪里remove是要还
是不要？而还有个问题，sample在rna later中放置久了会不会造成sample丢失？ |
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x*p
发帖数: 6 | 35 顺路问一下，order的primer的时候一定要PAGE purification吗？ 谢谢 |
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y*******t
发帖数: 1 | 36 One research scientist (project leader) position in molecular biology is
available in Qingdao Institute of Bioenergy and Bioprocess Technology. We
welcome those who are working in the field of enzymology, proficient in
protein mutation design, expression and purification. If interested, please
send your CV to z*****[email protected] and y***[email protected]. More details
can be found in the institute webpage www.qibebt.cas.cn. |
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d******n
发帖数: 29 | 38 Biotechnol Appl Biochem. 2010 Feb 11;55(2):63-72.
Title: Prokaryotic expression, purification and characterization of a novel
pro-apoptosis protein hPNAS-4.
Link: http://www.ncbi.nlm.nih.gov/pubmed/19912110
Please send it to d******[email protected]. Thank you so much for your help. |
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a*******e
发帖数: 245 | 40 if you add lysozyme in lysis/purification, most probably it is the lysozyme.
道lysozyme没被洗掉吗？按理
lysozyme不应该结合Ni柱啊，难道lysozyme粘住我要的蛋白 |
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n***e
发帖数: 180 | 41 Dynalbeads mRNA purification kit is very good. |
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a***e
发帖数: 1010 | 42 yeah, you need refolding most of time.
An alternative method is adding some sarkosyl during purification, which
solublizes your protein into the supernatant. |
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T**********t
发帖数: 1604 | 43 看了一下Qiagen网上的faq，好像不行，虽然没提MinElute，可是估计道理一样的：
Are QIAprep and QIAquick Spin columns interchangeable?
No. While columns from the QIAprep Spin Miniprep Kit and the QIAquick PCR
Purification- and Gel Extraction Kits are based on silica-gel-membrane
technology, each is designed to work optimally within its own kit format. In
addition, the binding capacity and DNA recovery size cut-offs of the
QIAprep and QIAquick Spin columns are different. QIAprep Spin columns bind
up to 20 ug of plasmid DNA up to |
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a***e
发帖数: 1010 | 44 separate nuclei and cytoplasm by 0.5% NP-40/PBS for 5 min on ice. then
standard purification of RNA.
there are also kits for these, just google. |
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s****a
发帖数: 436 | 45 Purification of biotinylated proteins on streptavidin resin: A protocol for
quantitative elution。
For elution of biotinylated proteins, 500 mL
release solution (2% SDS, 30 mM biotin, 50 mM phosphate,
100 mM NaCl, 6 M urea, 2 M thiourea, pH ,12)
were added to the resin and incubated for 15 min at RT,
followed by incubation for 15 min at 96C. Afterwards, the
resin was pelleted by centrifugation for 5 min at 16 100g |
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e***l
发帖数: 33 | 46 发个小广告，投条给我
Positions: research associates 1 and 2 (RA1, RA2), production associates 1
and 2 (PA1, PA2).
Location: Frederick MD. (no relocation package)
Company: top tier in biotech
Degree: BS/MS + 0-4 years experiences
Area: immunology assay development, (or) mammlian cell culture, (or) recombinant
protein/antibody purification
Has huge career development potentials.
Has very specific requirement. So don't feel sad if I tell you it doesn't
fit you. |
|
M**a
发帖数: 4816 | 47 Postdoctoral and medical fellow positions are available to participate in
ongoing studies of molecular and cellular neurobiology in developmental and
aged related brain disorders as well as clinical biomarker searching/
validation in Alzheimer’s disease. Candidates with a strong background in
molecular biology, including molecular cloning, vector design and
construction, enzyme assays and purification or cell biology, including
primary cell cultures of neurons, glia, endothelial cells and stem c |
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c*********r
发帖数: 1046 | 48 Thanks a lot!
Do I need to purificate the product of anneal before ligation? |
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s********n
发帖数: 2939 | 49 理论上可以这样：
构建两个带有不同purification tags而且可以相容的载体，如6xHis tag on pET和
Strep tag on p15A or dual vector。让这两个monomer coexpress，用Ni-NTA和Strep
tag的亲和柱顺序纯化即可。 |
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h****6
发帖数: 229 | 50 I used reversible avidin column for in vivo biotin labeled protein
purification. Elution was done with biotin. Worked pretty well. Forgot which
brand. Maybe Promega. |
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